XB-CLONE-3057896
Clone Name: IMAGE:7847534
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531951 [+]
taagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaaatccaaaacataccaggttatg
caggagtgcgaacaatctggaacaacagccccttctgtgttcagccagaacaggacgggcggagaaacagcctttgacaagccaaaggaaggcccagcga
aaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcattgtgctgcgcacatcat
gcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttcattgctcctatggtcgggctgt
gctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaacaaaggagccaaatatgatttttt
agatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttaggctttggctggaccaaatcaga
acacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggtgatatggttcagccctggcatt
tttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgctttaattgttgcattaaatgtaaaattagga
cttttcccgttaggtactcatccctaacaaactaagggctgataatctgagcaacctttataggctcaactgt
3' EST: DT531950 [+]ttttacagactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaacaaaccatataagaacatagcgacacaagaatgatcagggctaat
acacaacacacatttacttctcagtgatgctgtgactgggtgctctgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcaga
agcctagtgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatag
aactaactggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcactta
ttgaatagaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttgga
gcctataaaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcag
gtgatccctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccagggctgaaccatatcaccctgatgatgg
ggagtaggtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctgta
agggtttggattttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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