XB-CLONE-3057738
Clone Name: IMAGE:7847835
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532056 [+]
ctaaagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcac
caagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcatt
cagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatc
ccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaa
ctatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacac
tccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgacc
acttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagac
cttcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggactt
cagaagattgaagagatctttaaacagcagga
3' EST: DT532055 [+]tttagaggtgatattttggttttattgtctccaggcaatcattgggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctt
tatcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccaccca
catccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggt
gtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatga
gtttcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtc
aacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcct
ctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatct
tctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccag
gaaggtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgccaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: