XB-CLONE-3057712
Clone Name: IMAGE:7847933
|
Gene:
rpl7
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT532073 [+]
ccatcgattcaattcggccgaggccttttaaaaaggcgaaagcagtttgcagctgcaaagctcaagagagtcaaaaagatcgtggctgaaaaaaagcttc
gcaaacagaagaggaagatcatttttaagagagctgaaggctactacaaggaatacaggcaaatgtacaggaaagaagttcgtctggccaggatggctcg
taaagctggaaacttttatgtacctgcagagcccaagctcgcttttgtgatcagaattagaggtattaacggagtcagccccaaggtacgcaaagtactg
caacttctccgcctgcgtcagatcttcaatggaacctttgtaaggcttaacaaggccagcataaacatgctgaggctggtggagccctatattgcatggg
gttacccaaatctgaagtctgtgcgccagctgatctacaaacgtgggtaccttaagattaagaaccagcgtatccctctgactgacaattccctcattga
gaaacaccttggcaagaaaggcgtcatgtgcgttgaagatctgatccatgaagtctacaccgttggcaaaaacttcaaggcagcaaataacttcctgtgg
cccttcaaactgtcctctcccaggggaggaatgaataagaagacaacacactttgtggaaggtggtgatgctggcaatagggaagaccagataaacaggc
tcattagaaggatgaactaaaggttttcccatgatgccttcaagtctgtctgttaataaaatctggtttaaaacaaaaaaaaaaaaaaaaaa
3' EST: DT532072 [+]gttttaaccagattttattaacagacagacttgaaggcatcatgggaaaacctttagttcatccttctaatgagcctgtttatctggtcttccctattgc
cagcatcaccaccttccacaaagtgtgttgtcttcttattcattcctcccctgggagaggacagtttgaagggccacaggaagttatttgctgccttgaa
gtttttgccaacggtgtagacttcatggatcagatcttcaacgcacatgacgcctttcttgccaaggtgtttctcaatgagggaattgtcagtcagaggg
atacgctggttcttaatcttaaggtacccacgtttgtagatcagctggcgcacagacttcagatttgggtaaccccatgcaatatagggctccaccagcc
tcagcatgtttatgctggccttgttaagccttacaaaggttccattgaagatctgacgcaggcggagaagttgcagtactttgcgtaccttggggctgac
tccgttaatacctctaattctgatcacaaaagcgagcttgggctctgcaggtacataaaagtttccagctttacgagccatcctggccagacgaacttct
ttcctgtacatttgcctgtattccttgtagtagccttcagctctcttaaaaatgatcttcctcttctgtttgcgaagctttttttcagccacgatctttt
tgactctcttgagctttgcagctgcaaactgctttcgcctttttaaaagg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: