XB-CLONE-3057625
Clone Name: IMAGE:7847893
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Gene:
epb41l2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532131 [+]
cactgggcggtaacctctttaatggttgatcacttatatattgtaataaaataactatttcttgttatgctttatagctggatgacagtggtgacttaga
acctggtgtattactaagtgcacaaaccatcacatctgattctgttcttaccaccacaactacacacatcactaaggtagacatgaggctatgattgtaa
ttttttttgtttgtttttgcagagtaacattctgatgttaaagaagctgctaaaagacccaggacctttcttctgtaaatttttcacaacactgttgtgt
tcagactctcttgccttttagcagtgatagaagggaatgcttctcaagacaaacctttcaccccatacttattataagccttgattaagctgacatttta
tagtgtttacagaaaatatatgcccctgtcctgctggggtttacccctttctgcacagggccaggtaaccgtcttgttttggaggtaaccttggtaaccc
actcgagcactcaaaccttctcaagtacagagatacattacattgcagacattgccctggttagaactgaacctaggacctccgtgttgaagcactaccc
cacatgctgtgcctatagttgcctgtagaagggaaaactttggaaaaagagtgttggctctatgttgtttattgtttgcgggatctttaaacgtaaacct
tgattaaacatcttacattcatgataaatatttattgtatagaagattttgttttttaaattaaataaatcggttgtgactagtttaaaaatactaaagc
tgtgttactattaattacttaatggatctcaatcagcag
3' EST: DT532130 [+]tttttttgaatagaataaacaaactttatttttaaaagtattacagaaagcaaaatattcttaatacagctggaagcaggtataaatctatttggcattt
taactatacaaagggtcaactgtaaatcaaaacaaaaacagagacaaatacagttcttttagtggcaaaagtgtgtgtggttctcaatgtttgcaatagg
actgtaacggcatccattttgcattataaaaacctgcataaaccgttataagatttttccccgtttttactacaggcaagccactttggcaatgtaaaaa
aaaaaaacacagattaaaaaaaaaaaagttttacatgtatattcaaattcaaagtaggacaaacgtaacaagctgccagaaaggtttcctccttttttgg
aacagtgatactccttttttgcatctttttctcctggaatgtttgatgtcttttttttccaatctattgtaactagcagaaaagttcagcacaaatatgg
cagtacaaaatgtagagaccacacaaggctggctgaacctctgcttactaatatgcacaaataaaatccccttacaaaattaaaaatttcctcattgaac
cctcctagtaacaaagaactaaaattggctttcacttttttatctctaaagtacaaatattggtataaaacaccagtgaaagtgaaatacattttgcaaa
tataggaaacgtgtaaaatgttttgtttttcaattactctaaagagtgaaatgacttgaataaactaagtctgaaaagtgccatttccaggggcttaaac
tgacacattaataaagaacaaagagaaactgtgctagtgacaaggattttgaattctctacaactctacaatctggaaaaatggttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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