XB-CLONE-3057604
Clone Name: IMAGE:7847943
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Gene:
ubb
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532145
[+]
cacaatagaaaatgtcaaagcaaagatccaagacaaagaaggtatccctccagaccagcagaggttgatatttgccggcaagcagctggaagatggccgc
accctttctgactacaacatccagaaagaatccaccctgcatcttgtccttcgtctgagaggtggaatgcagatctttgtaaaaaccctgactggcaaga
ccatcaccctggaggtagagccaagtgacacaatagagaatgtcaaagcaaagatccaagacaaagaaggtatccccccagaccagcagaggttgatctt
tgcaggcaagcagctggaagatggccgcacactttctgactataatatccagaaagaatccaccctgcatcttgtccttcgtctgagaggtggaatgcag
atctttgtaaaaaccctgactggcaagaccatcaccctggaggtagagccaagtgacacaatagagaatgtcaaagcaaagatccaagacaaagaaggta
tccctccagaccagcagaggttgatctttgccggcaagcagctggaagatggccgcacactttctgactataatatccagaaagaatccaccctgcatct
tgtccttcgtctgagaggtggaatgcagatctttgtaaaaaccctgactggcaagaccatcaccttggaggtagagccaagtgacacaatagagaatgtc
aaaacaaagatccaagacaaagaaggtatccctccagaccagcagaggttgatctttgccggcaagcagctggaagatggccgcaccctttctgactaca
acatccaaaa
3' EST: DT532144
[+]gagaaataaatgctttatttagaacatacaagattactgttaaggaacgtgaacaaacaaatcacatgaagcaaagtcttttttcactttaacaaccacc
tctcagacgaaggacaagatgcagggtggattctttttggatgttgtagtcagaaagggtgcggccatcttccagctgcttgccggcaaagatcaacctc
tgctggtctggagggataccttctttgtcttggatctttgttttgacattctctattgtgtcacttggctctacctccaaggtgatggtcttgccagtca
gggtttttacaaagatctgcattccacctctcagacgaaggacaagatgcagggtggattctttctggatattatagtcagaaagtgtgcggccatcttc
cagctgcttgccggcaaagatcaacctctgctggtctggagggataccttctttgtcttggatctttgctttgacattctctattgtgtcacttggctct
acctccagggtgatggtcttgccagtcagggtttttacaaagatctgcattccacctctcagacgaaggacaagatgcagggtggattctttctggatat
tatagtcagaaagtgtgcggccatcttccagctgcttgcctgcaaagatcaacctctgctggtctggggggataccttctttgtcttggatctttgcttt
gacattctctattgtgtcacttggctctacctccagggtgatggtcttgccagtcagggttttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: