XB-CLONE-3057486
Clone Name: IMAGE:7847811
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Gene:
svil
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532223 [+]
caaccgtctcggtggatgtctggcatatccttgaattcgactacagccggctgccgaagcagagcattggccagttccacgagggagacgcctatgtggt
aaaatggaagtactttgtgagcacaacagttggacagcgccagaaaaccgagcacgtccggatagcgggtaaagagaagtgcgcctacttcttctggcag
ggacggaactcgcccgtaagtgagaagggaacctcagcgctgatgactgtggagcttgatgaagagcggggagcacaggtccaggtgcttcagggaaaag
agcccccctgtttcttacaatgtttccagggaggaatgattgtgcatgctgggagacgggaagaagaggaagagaacacgcaaagtgactggcggttgta
ctgtgtacgtggggaggtgccggtggaagggaatttgatggaagtcgcttgtcactgcagcagcctgcgctccaggacctccttgatactcctcagtata
aataaagcgctcatctacctttggcacggctgcaaagtccaaatccatgccaaggaagtgggacggacggcagccaataaaataaaagaggagtgccccc
tggaagccgggctacacagcagcagtaaggtgaccatatatgaatgtgatgaaggctcggaaccccccggattctgggatgcgctgggcaggcgagaccg
taaagcgtacgactgcatgttgcaagatcctgggaaatttaacttcacgccacgactgttcagcctcagtagttcatcgggagagtttacagcatcagaa
atcctgtatccttncaggcatccgaatgtggttaactccatgccatttttacaagaagacctg
3' EST: DT532222 [+]cattctgaagcattttaattcaggttctgatatcacaaaatacatgttttaaagtttacatcagggtattagaaactgagatgtataatattataaagta
caaatattagaggctttccttaaactgccttcaaaatgcaggctaaatgacctgtatataaactgtacacaaaaacaaggcactatacacttttattata
tgaacccttcaggtcagacatttactcacaaagtggggccaactcacacgctgggaaaaaaaatgtaaaaaaacaacaacaaaaaaaaggaggcatgaag
taaatgatgatcttaaagcttagcccttttggccactagagggcgccgtggtgaccaagggtaccagaacgcttactgctttgttcacccgagctacaga
cgcagcgttacttacttaacatgcaagttgcaaacatgggatttcccaaaatgcacatggaggtaactacaggtggcaacgcagctcagagtaggcaacg
gggcgtaactaggtttgtgtaatgacacacaaatcttacacagtgagtatattgcatagttaaatacagtgtttatataaataaaattctaaatgatcga
accaacaaatacaagacatttacaaaagagaaccggtatatttgcggcgacggaaaaaaaaaatacgggtgaattcatgttattaagtgcacagaagagg
attgatatttccgaacgtcctctgagaagtgaaggcttctgattggttctcttgtacatttacaaaaaagggaaaaaaaaaaaaaaaaaaaggacacaat
tcaacaggcagcactgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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