XB-CLONE-3057477
Clone Name: IMAGE:7847955
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Gene:
shmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532229 [+]
ctacctctttatcaccactgggggcgctgttcgcagtgatggagcagcagcaagcactcatgtacaaatgcagaaatgtttggggggttgtaaggtatag
cttcccctttaaagcgattgtaagcagagtatttccgttctttagagcagtgacgttgggcggctgcctgggattatgggggggctgtgggtcactgctt
tagagagaggcccccgtatagacctgctcactgtgcatacctcactataggctgtattattatttttctaggatcgctgttagacctactttttatttgt
ggttgttgttgcacaattccttgcacataccaccccccccctcccatttaggctcaggattagacacccctctttcccctgctcccaggattctgcccat
acggtgctgctcttctgcgcggggccctacccccatacattctgtgcgttggagcgacccccccgtgtcccacttgtcctgtgtatatggctggtttggt
ccctttgtccttaccctactaccaggtgtgctaactagacataggagcgattggagtgtttgtagttttattttagcccccccccccatccagagcacag
gttcctgcctacagattgcgggggtcatttataaacactgggcaaaattgcacccaatcagatgtttgattttattgttctgacattgaaagctaatcac
tgattggttgctatgggttactgcccaggtgcaacgttgcagttttgctccgatactgcccctcccgaaagccctacattctctctgtggctgttgttgt
tgcacatagcgggcgccaatccgtcttatacttgacctttacctttga
3' EST: DT532228 [+]ttttttggaggttaaaagtgtaattctttgacatcgttaatcaatattataacaaaatgacaaaaaaaaactatgataaaatatgcaataagttaaaata
aaacaactgaaataaaaagaacagcatgaaacaaataactcatctcagtgataatatagtttgtaggttacaatactcatgggtgcagggggcggggcaa
caatgccacaaacagctgcaccaagatggcggctagggccgtagtcgacatggccggtgattacagcaagatggctgaaaacaacagcgacaaaagaaaa
aaaaaaaaaaatgtccaacagtgactggtccaagatggctgaatgactgatgtagttcctggtccaaaggtaaaggtcaagtataagacggattggcgcc
cgctatgtgcaacaacaacagccacagagagaatgtagggctttcgggaggggcagtatcggagcaaaactgcaacgttgcacctgggcagtaacccata
gcaaccaatcagtgattagctttcaatgtcagaacaataaaatcaaacatctgattgggtgcaattttgcccagtgtttataaatgacccccgcaatctg
taggcaggaacctgtgctctggatgggggggggggctaaaataaaactacaaacactccaatcgctcctatgtctagttagcacacctggtagtagggta
aggacaaagggaccaaaccagccatata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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