XB-CLONE-3057387
Clone Name: IMAGE:7848059
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Gene:
cdc42
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532288 [+]
ccaactgtgctgaccttcacaccagaacatttcagccatgcagacaattaaatgtgtagtcgttggtgatggtgctgtgggtaaaacatgtctccttatt
tcttacacaacaaacaagttcccttctgagtatgtaccaacggtttttgataattatgccgtaacagttatgattggaggggaaccatataccctagggt
tatttgatactgcaggacaggaagattatgatagattacgacctcttagctatccacagacagatgtgtttctagtttgtttctccgtcgtgtctccatc
ttcatttgaaaatgtgaaagaaaagtgggtacctgaaatcactcatcactgtcccaaaactccatttctgctggttggcacccagatagatttaagagat
gatccttcaacaattgagaaactggcaaaaaacaaacagaaaccaatcactccagagacagctgagaaactggcccgtgacttaaaggcagtaaaatatg
tggagtgttctgcacttacgcagaaaggccttaagaacgtatttgacgaagcgatattggccgccttggaacccccggagcccaagaagaaacgcaagtg
taggctgctatgaatattcctccgtggtccccttttttgcagtgctggtgtcgacgtcaaactaaaagcaaagttaaaatcaaaactaagtataaactgt
taaatttgttttgcaataatgccagatgctctgcactcattaccattcagaatagtggggtgtctgaagacaaaacccagcaacctattgaattcagtat
aaatattagtagagcgaatatgtatctgcagaaaaaaacactgattactagttttattcatttttt
3' EST: DT532287 [+]ttttttgaaggtattttatgtttaatacatctgaaaagtatgcatatacaaagaactcagacatgaaacaaagcatgggttcaacactaatgagtctaca
tgtgttatccaggaggatggaatatatttcctacatattaaaaattctgaaaaaaagtgggttctggtaaagtccttattaacactggtgtctattttta
cattatgttttagacaggaaccctaaaataaggattcttagaccttgcatgcaaaatgtacataaaacgggatttttttgactggtccctcatgtatcaa
actaaacaaaacagacaggaaaaacattaggcacatgtatagtttgtcagaactttaaggggtagctaactactagtatgtttaactggaatcaaattca
attatccggattggaaaacattttcaaaaatctaaattttggtttgatataacctgccaagacactgtcaaaaagtacatcggcctccttgagatcacaa
aagcttccaaggcattgctctgcaatgcagctgtagccccttaaagaacaagggaattcaaagatgtaagaagtatcatttactggcaaatatttggtta
ctcatggtcactattccctagtgcttcccaaatgaagcagctgacccaaaaacagaagtagcattctgtgtgcaacgctacatgccgcagtggctagaga
tataaaagccaatggctttttctatatttgggctctgggagacctttaatagtcctgagattcaatcgttttatatttaaacctgttctctagccaactt
gtgtactttttgcaggagagcagagaaggaattcctgattcccagcaacttgnggaaagagttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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