XB-CLONE-3057248
Clone Name: IMAGE:7847833
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Gene:
cox20
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532380
[+]
ggatttctcctgatttaggttcaggaaaaattacacaacttgcttgttccttagggatttcatcagggttgccacactagcctttatttaaaactactgc
caatactgtcactttgtggcctgtagtatttaagcattgtgggtatgtacccacttgcacttaacaatctacatgtgttcctcttatgtggcatctgctc
cagtgtgagaggacatcagtgctgaaggcagaaaacaggtttccctttaccccacagggcagaccagttggctccagtttagtgccatgaggcaaaagaa
ttatggactgcactgtgtaatacatcctatgtagtgctgtacccaattgtcaacgccacagggcatattttatagaaaaaaaatataaaggtggtggtca
tttaacagttactgaaaaggtgtggtttattgttactgccacaaactcctatattctttaaatgcaggttttggaaaggtgtttagcatatgcattggaa
tcctgtttttgatgcggaaaatcaggtttttgagtcagttgtgtttttacgcgtatttggatctatactgctgccttcaaatagtatcctatttttaatt
ccttcttgtaacatcttttgctggatccttacttttgcattattgtatctgcagtgcatcctgcaaattaaaacaaaaatactgttattgattctgccaa
aaaacttttaatactttaaaacttttaacttaattttaaacaaatggtaagcttagaatagctaagcttgtttgagtcaatgaatgctgttcttgtatat
acaccaccaac
3' EST: DT532379
[+]caataaaaaggctttattttacaattattttaattcacagtgaaaatgtattcagaatggaatttcttcatgctgggcagtccatcttcagctttatctg
ggtttctgttgaattagttcttttcataatgcaggtttttaagctgtccaaccatttagcaaacagaaaactgccatttctggctcagagtgcaggaatc
agaaatgaaatgtgagcgcatacttacactatttccctagttagtggcaatcacaatcaagactacatggcatcaagcagttgtgttccctattgaaatt
ttttctgcttcattcagtgcccattgagcaaactactcattccaggcacaccttggtttgtacacatttgtcagtatggcctgctattcttggggtttat
tagcatttggcacttagatgtacatactaaagatgttggtggtgtatatacaagaacagcattcattgactcaaacaagcttagctattctaagcttacc
atttgtttaaaattaagttaaaagttttaaagtattaaaagttttttggcagaatcaataacagtatttttgttttaatttgcaggatgcactgcagata
caataatgcaaaagtaaggatccagcaaaagatgttacaagaaggaattaaaaataggatactatttgaaggcagcagtatagatccaaatacgcgtaaa
aacacaactgactcaaaaacctgattttccgcatcaaaaacaggattccaatgcatatgctaaacacctttccaaacctgcatttaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: