XB-CLONE-3057214
Clone Name: IMAGE:7847980
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Gene:
MGC75753
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532402 [+]
agccaatggttataccaaaatataaattctctcttgactcatgatttcattttacaatgtacaatttcagagaccggagcgagaaaatgtcataaaagaa
agacagaaatgacaataagagctttttgccccagatggtattctacattcaccttctgcaacaagacggtacatttttggcagtattacagttttaatgt
ttatggaatgaagatgaaacattaacgcaaatcaaggcagaagagggacacatacgtaattgtacaattcctgatttattttttagctgtttaactagaa
actgtcattatgctgtaatttttggaggggctattccggccaattctaagtgcatccttgcagcttgcatggttttcaataggcttgccccctgctgcgg
ctccttcatacaatacacagatggacccatcctcaccaatgcgataggagacttcaaaagggtcaacccaaagagtcagctcgctggggagaagctggaa
cagtctctggtggctgagcccaatgtgacctgctgcctgtcctatcaaggggtccattttgtggttaattctcaagcaacggtaccctgatcctttgaaa
gggttctgcggaaaccagtggtgtttataatgatccttcatggcctcctgtaggacctggcagaagtgctgcagctgcctctcctctatgaccccggttg
ttttcagcanacttgtgataaacgcagcagctgttgagatctcagtcatcattgtggcatataaggttgaatcctcacagctatataaacatcaacagca
gcgggtctctctcttcgatcgtataggcaggacagaat
3' EST: DT532401 [+]gacatcggacggagtgagagggctagctctggcctgcttcccccccttcagcgaaacactcgggggtagaaagttgaggactctggctgccggagatcac
aaaggaatcgcgtacgattctgtcctgcctatacgatcgaagagagagacccgctgctgttgatgtttatatagctgtgaggattcaaccttatatgcca
caatgatgactgagatctcaacagctgctgcgtttatcacaagtttgctgaaaacaaccggggtcatagaggagaggcagctgcagcacttctgccaggt
cctacaggaggccatgaaggatcattataaacaccactggtttccgcagaaccctttcaaaggatcagggtaccgttgcttgagaattaaccacaaaatg
gaccccttgataggacaggcagcaggtcacattgggctcagccaccagagactgttccagcttctccccagcgagctgactctttgggttgacccttttg
aagtctcctatcgcattggtgaggatgggtccatctgtgtattgtatgaaggagccgcagcagggggcaagcctattgaaaaccatgcaagctgcaagga
tgcacttagaattggccggaatagcccctccaaaaattacagcataatgacagtttctagttaaacagctaaaaaataaatcaggaattgtacaattacg
tatgtgtccctcttctgccttgatttgcgttaatgtttcatcttcattccataaacattaaaactgtaatactgccaaaaatgtaccgtcttgttgcaga
aggtgaatgtagaataccatctggggcaaaaagctcttattggcatttctgtctttcttttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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