XB-CLONE-3057177
Clone Name: IMAGE:7847888
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532426 [+]
agatgcagagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgat
gcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatga
aagaagtcttcagacgcttctgtgagggacttcagaagattgaagagatctttaaacaagcaggacaccccttcatgtggaatgaacatctgggttatgt
ccttacctgcccatcaaacttgggcacaggcctgagaggaggtgtccatgccaagctccccaacctcagcaaacaccccaaatttgatgagatactgggc
aggcttcgtctgcagaagagaggcacaggtggtgttgacactgcagcagtaggaggtgtctttgatatctccaatgctgataggctgggcttctctgaag
ttgaccaagtccagatggtagtggatggtgtgaaactcatgattgagatggagaagaagcttgagaagggacagtccattgatgatatgatgccagcaca
gaagtagatgacaggggctagagacagagcctaaacaccctaacccttaccaccatcaaaggggacaggagaccccattaagatcattagaggtcaaaaa
atggccatctacaggggaatacataagtaatggatgtgggtgggataatctcagaaatgtagtaggaagatccagcacaagaaaacaaaaacttcanaat
caacataaaagagaagataaatctaattctct
3' EST: DT532425 [+]agaggtgatattttggttttattgtctccaggcaatcatgggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatc
agagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatc
cattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgttt
aggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagttt
cacaccatccactaccatctggacttggtcaacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaaca
ccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctca
ggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctg
aagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaag
gtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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