XB-CLONE-3057156
Clone Name: IMAGE:7847842
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Gene:
tmem182
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121614 [+]
CAAAAACAGAAATATTTTTTAATTTAAAAAAAAAAAAAATCTCTTTGTTTTTCCACATATTTCTAATCTATTTATTAACCTAAATGAAGATGAAGCTAAG
TATTGGCATATTTTTTGGTGGTCTCTTTGGTGCATTGGGGATTTTAATCTATCTCGTAGCCTTTGGAAGTGACTATTGGCTTCTTGCCAAGGAAATTGAG
AAATGCTCAGAAAACCAAGAAACTGGGGATAATGCAACCCACTCGGTTCTCTTGCACCATGAAGGATTTTTCTGGAGATGTTGGTTTAATCATGCTGAAG
AAGAAAACTCAAACACCATGGAGAATTTCTGGTTCACTAACCAAGCACCAACTAAGAACTGCACCCATGCATACCTCTCCCCATTTCCACAGAATAGAGA
CAACAGTAACTCCACCTCGTATCATTCTGCACTTGTATACCGTGGCTTCTGGAACATATTCATGCTTCTTGGTGTAGTGACAGCTGTAATTGGGGGATTC
CTCATTATCTGTGCTGCTCCCTTCACAAACCACAGAATTTATAAAGCAGGAGGGGGCCTATTCATAACATCTGGAATTCTCTTTGCACTGGTGGTTGTAA
TGCACGTCTTTTGGGTACAGAGCGTATCTGATATCAAGGGTTACACGGACACCAGACAGCAAGATTGTTCCCAATTCACCGTGTATGTTAGCTTTGGCTG
GTCATTCATGTTGGCTCCATTTGGAATATTTTTCTGTTTATTTGCAGGAATGCTTTTTCTGCTAGTTGGACATACTATACATGTACATACCAAGTAACAC
AAAAAAACAGAAATAACTTCCCAGTAATTTCTTTTCTATATCTCTCAATCTGTAATAATAGGGTTTTGTAAACATTTTAATCTTTTATTATGTCAATGTC
AGACCAAATCATAGAATTAGAATGTGTTTATTCTGTATTTAAATGTACTATATAAGCAAACACATAACTTATTTTTTTCTGTTCTCTTCAATGTGCAAAA
TACACAACATCCTTGGCAGAGAAAAAAAAAAAAAAAAAA
5' EST: DT532440 [+]caaaaacagaaatattttttaatttaaaaaaaaaaaaaatctctttgtttttccacatatttctaatctatttattaacctaaatgaagatgaagctaag
tattggcatattttttggtggtctctttggtgcattggggattttaatctatctcgtagcctttggaagtgactattggcttcttgccaaggaaattgag
aaatgctcagaaaaccaagaaactggggataatgcaacccactcggttctcttgcaccatgaaggatttttctggagatgttggtttaatcatgctgaag
aagaaaactcaaacaccatggagaatttctggttcactaaccaagcaccaactaagaactgcacccatgcatacctctccccatttccacagaatagaga
caacagtaactccacctcgtatcattctgcacttgtataccgtggcttctggaacatattcatgcttcttggtgtagtgacagctgtaattgggggattc
ctcattatctgtgctgctcccttcacaaaccacagaatttataaagcaggagggggcctattcataacatctggaattctctttgcactggtggttgtaa
tgcacgtcttttgggtacagagcgtatctgatatcaagggttacacggacaccagacagcaagattgttcccaattcaccgtgtatgttagctttggctg
gtcattcatgttggctccatttggaatatttttctgtttatttgcaggaatgctttttctgctagttggacatactatacatgtacataccaagtaacac
aaaaaaacagaaataacttcccagnaatttcttttctatatctctcaatctg
3' EST: DT532439 [+]ctctgccaggatgttgtgtattttgcacattgaagagaacagaaaaaaataagttatgtgtttgcttatatagtacatttaaatacagaataaacacatt
ctaattctatgatttggtctgacattgacataataaaagattaaaatgtttacaaaaccctattattacagattgagagatatagaaaagaaattactgg
gaagttatttctgtttttttgtgttacttggtatgtacatgtatagtatgtccaactagcagaaaaagcattcctgcaaataaacagaaaaatattccaa
atggagccaacatgaatgaccagccaaagctaacatacacggtgaattgggaacaatcttgctgtctggtgtccgtgtaacccttgatatcagatacgct
ctgtacccaaaagacgtgcattacaaccaccagtgcaaagagaattccagatgttatgaataggccccctcctgctttataaattctgtggtttgtgaag
ggagcagcacagataatgaggaatcccccaattacagctgtcactacaccaagaagcatgaatatgttccagaagccacggtatacaagtgcagaatgat
acgaggtggagttactgttgtctctattctgtggaaatggggagaggtatgcatgggtgcagttcttagttggtgcttggttagtgaaccagaaattctc
catggtgtttgagttttcttcttcagcatgattaaaccaacatctccagaaaaatccttcatggtgcaagagaaccgagtgggttgcattatccccagtt
tcttggttttctgagcatttctcaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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