XB-CLONE-3057017
Clone Name: IMAGE:7847854
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532532 [+]
cccagccacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaa
atccaaaacataccaggttatgcaggagtgtgaacaatctggaacaacagccccttctgtgttcagccagaacaggacaggcggagaaacagcctttgac
aagccaaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttcta
ctcattgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttc
attgctcctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaaca
aaggagccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttag
gctttggctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggt
gatatggttcagtcctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgctttaattgt
tgcattanatgtaaaattaggacttt
3' EST: DT532531 [+]tttttcgcttgtacagactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaaaaaaccatataagaacatagcgacacaagaatgatca
gggctaatacacaacacacatttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccc
cgagcagaagcctagtgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgacc
aatcatagaactaactggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtca
ttcacttattgaatagaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattcta
cagttggagcctataaaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaat
taaagcaggtgatccctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccct
gatgatggggagtaggtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaaaagaggtgttctgatttggtccagccaaagcctaag
gacctgtaagggtttggattttagaaaatacaaattctgtattcacttcctccattttatatataggaaatctaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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