XB-CLONE-3056894
Clone Name: IMAGE:7847866
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Gene:
pfkfb4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532614 [+]
taatgagtcagtaaatgaaggggaaatctagtgcaggtatttcttaattgtatgcttccaggtcttgctgaactacaacatctgttgctgactggctgtg
aattatgggagttttagttccgcagtagcgagattgcagggaatgtctcgttactctaagctgttgtataaaaagccattctattgttccctccccaggt
cactgatatactgtatcttattacttgggggtgcatggtacatacaatgtggcttcttgttgccagcctacaggaaataatctctaagcaccttgtatgc
tcccagtagcatctgtttgaagggtcccacaggtccaagcgcaccattgcccctcagcaacactagagcagaagactggtctgtttttgagatatatatt
ggcagagccagggccaccatcactaatcatggggtgccacacttctaagttagcagggttctcccccaggggaaccccaaatattagcccattgctcacc
tgtgtggtggccctgccatcaaatcgaatgggggccaaatgaaaacgaataccctactcacgcatggtcccaaccctgatccaacttaccatgcttcatt
atgccaaatcccttcccatgatctgccaaaccctgtccatttccctacaagtcctactccttcaccgacctccactgccatagggactgcagtacccatt
gtaccccctgatgggatccctggtaatccacacattgagtttctctgcatttatccttatttttgcactacttttctgctgnggaacagactaacccatg
ttccatggcctctgaaggcaataatcgagaaaggngaggaggggtcaggctcattcaatttttacaaaacaacctgctctaaatca
3' EST: DT532613 [+]catacaagataaattgtatttcactttgcattcaagttctaaatttaaaatgacaaatttatacatgaaaatattgccttctctttacactttctagaga
acatttcacagaaaatttagaaaggctgaactctgtcacagttgtgttagctttgcctcggggtcagaaatggagaagccataaaggaaagaaccaaggt
gctgcactcggaaggatgtgtgtgccccgtgctacagcgcagacaggaagggagatcagaatataaaggtgcagataggtctgtgcatggattggactgg
catttacacaataggagtgaaccatctccagtgttggtatcacttcccagttcaaataaaatagccatttacaaccgtcatatagattgaaagacagcag
catgaaaacaaagtgctatttcagtgctgaagaaggggatacttggggttcaaaccgaatgcgccaaatttctgccttaatcagaatcacgacccttcag
agtgtttggccatagaaagcagacaccgggctgcacacaaatatagaaagcgcaagtgaatcaccatcatcccgagagcctctgcacatacagatgaact
gtacatattgcacattgtaactcacagtcccagtagcaagtgcaggagaacaaaccgtacagaatataaaataaaaaggctattggttatttacatagaa
gactttgtgttggaattgtattcacctgcaaataaacctttatcagtgcagaataacccagaatcctgtgccacaataactcactgggaaagccatttgt
aaggagcagcatgtatgtgttggaggacaaggatcaaacgcaagccagcttcttcccttgcatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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