XB-CLONE-3056828
Clone Name: IMAGE:7847824
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Gene:
ifit1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532658 [+]
catctgcttaaactggagtgccacttcacatngggcttttttcaaagaagaagctgatgttgacaatatagaagaaaggcttcatgatcaaattgcattt
ttaccttctacacacagacataggcttcataatttattggcctatacaagttatttgaaaggagacaaccaagaagcaatcagacagcttcaaaaagctg
aagagcacctgcaaggaacacagactgctcatctggatatcaaaagagccgtgacctacagtaattatgcctggctgtactaccattccaaccagtttag
caaaactcagttttatttagaaaaagtagaagccatttgcaagaaatgtgagtcttctccagaacatgatattcttcttactgagagatatggagaacag
gcctgggctttgttaacattatatgggaagtactgtgaaagagctaaagagtgttttgagaaagcgctagtgctagatcctgacaatcccgagctcagct
caggatatgccatagtgatatatcgcttagaaagtcaacattacagagattatgggacagatgaaggtacatcattagagttgctgaaacgtgctgtgac
attaaatgaaaatgatactgtgattaaggctcttctagctttgaaatatgtatatttgggccaagctgaggagggaggaaagattatggaggaggctctc
atacaaactccagactccccatatttactacgatatgctgccaaattttacagaattgtaggaaagactggtgatgctattaccattctgaaaaaagccc
taaaccaaactcccacttcaagttctcttcaccaccagat
3' EST: DT532657 [+]ggataaatacagctttaattagatgtacaatattcactacagctgatagacaacaataaaaatacacaaatctactaattgtttcttgttatgaatatga
ataattgacaacaatgtaaataaaatcaaaacacatgaagaaagaaaatattcacataacttgctaatgccttgctaataaacagccctatttaacgtga
tatttaaattgaattcatttgttttaatactgtatagagcaaattatataagtatttcctgtatcaaattaagagtttgtagcctgttgctattatgttt
gtgtttataacatggttagtcaagttcttgacagaaaaaaatagcatttattttcaacattatttttccttctatagttccatgcaaagggctgtgccag
agacatgtgcaagctcagcaaggcattttgccattgaaggaaaaaaaaaacacctataagcagctaatctgttgagttgttactcactgagtctcatgca
acacaaagcactcaaataatcctcattatctggatcgcgttccagtgctttcttatagtaatcagttgcttgtttaatctctccatcctgctggtaaatg
aaccctaacagtccaaatccagttgcgtctgatggttcagtgattattttcctttctgccaacttttccaaattacttttggaaaagcgtcttgcatttg
tgttattcagaataagcaaagcttctttgtaatgccttatggcttctgattcagatctcatttgaaattgcttaaaaactgcataactc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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