XB-CLONE-3056790
Clone Name: IMAGE:7847780
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Gene:
trim63
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122073 [+]
GAGAGACCTAAAAGCTACGACTGAAAATAAAGAAGATAATTTGTCAAAATGGATTACAATTCAGAAATTATCCGGGAGAATAATCCCATGGATAGTCTTG
AAAAGCAGTTAATCTGCCCTATTTGTCTGGAAATGTTCAACAAGCCTGTGGTAATACTACCATGCCAGCACAACTTGTGTAGAAAGTGTGCCAATGATGT
GTTCCAGGCTGGTAACCCATATTGGTCTACAAGGAGTTCAGTATCTGGAGGGCGATTCCGCTGCCCAACCTGTCGTCATGAAGTTATCTTAGATCGTCAT
GGAGTATACGGTCTCCAGAGAAACCTGCTGGTAGAAAATATAATAGATATCTACAAACAAGACTGTACTAGTCGGCCTGAAAAGAAGGAAAACCATCCAA
TGTGTGAGAAACATGAAGATGAAAAAATAAATATATATTGCCTCAGCTGCCAGGTAGCAACATGCTCTATGTGCAAAGTGTTTGGAGTTCATAAAGACTG
TGATGTGTCTCCCCTCCAGAACGTGTATCAGAGTCAGAAGACAGAACTGAATAGCTGCATATCGATGCTGGTAGCGGGTAATGATAGAATTCAGTGTATT
CTTAGCCAGCTGGAAGAATCATGCAAAACTATACAGGAGAACAGTCAGAGTAAAAAGGAGTCTGTGAAAGAGATGTTTGAGACACTTTTCAGGAACCTGG
AGGAGAAAAAGACTCATCTGGTACAGCGGATTTCACAAGCTGAGGAAGAGAAGTTGAACTTTGTGCGTTCTCTCATTGTACGGTATCAGGAACAGCTCCA
GTCCTCATCCAATGTGCTACAGAATGCTTTTCAGGCTATGGAACAGCCTGGACCTGCTAACTTTCTTCTGGAGTCCAAACAGCTTATTAAAAAGCTAATG
GAAGCATCCAAAGGCTGCCAATTGGAGAAGACTGATCATGGATTTGAAAATATGGACCAGTTCACCCTGGATATGGAACATGTCAGCCAGTGTCTGAGAA
GCATTGACTTCACTCCAAACTGTGATGAAGATGAGGAAGATGATATAGAGGAATATGAAGAAACTGAAGAAGAAACACTTGAAGGGCAGCAATGATGCCA
GGGCAAGAAAAGCATGTGAGAACAGCGTGGGAAGGATCTACCATACTCTCCCTGAGAGTAGGGAAAGGGACATCACATGAGACTGGTGGAAAGAAAGCCT
GTAGGCAATGACTCAGGACTGCTGCTAATGATCAGAAGACTTGCATTCTAGGTCGTTATGATCCAGATGTATATATTTATTGTAATATATTTTATATTTG
CGATGGGTGCTGTGACTCTCTATTTGGGTCGATCCATAAGTCCTAGGGACTCTTGTTACCTGGAGGGCAGGAGGAAAGAAGTGGTAACACCACATGGTGA
ATCCCAATTTGTGTGATACAATTTCACTGCCTTTCTTTTGCATCTTAATAAATAAATTTTAGTGTTAATGAAAAAAAAAAAAAAAAAA
5' EST: DT532683 [+]agagacctaaaagctacgactgaaaataaagaagataatttgtcaaaatggattacaattcagaaattatccgggagaataatcccatggatagtcttga
aaagcagttaatctgccctatttgtctggaaatgttcaacaagcctgtggtaatactaccatgccagcacaacttgtgtagaaagtgtgccaatgatgtg
ttccaggctggtaacccatattggtctacaaggagttcagtatctggagggcgattccgctgcccaacctgtcgtcatgaagttatcttagatcgtcatg
gagtatacggtctccagagaaacctgctggtagaaaatataatagatatctacaaacaagactgtactagtcggcctgaaaagaaggaaaaccatccaat
gtgtgagaaacatgaagatgaaaaaataaatatatattgcctcagctgccaggtagcaacatgctctatgtgcaaagtgtttggagttcataaagactgt
gatgtgtctcccctccagaacgtgtatcagagtcagaagacagaactgaatagctgcatatcgatgctggtagcgggtaatgatagaattcagtgtattc
ttagccagctggaagaatcatgcaaaactatacaggagaacagtcagagtaaaaaggagtctgtgaaagagatgtttgagacacttttcaggaacctgga
ggagaaaaagactcatctggtacagcggatttcacaagctgaggaagagaagttgaactttgtgcgttctctcattgtacggtatcaggaacagctccag
tcctcatccaatgtgctacagaatgcttttcaggctatggaacagcctggacctgctaaacttc
3' EST: DT532682 [+]cattaacactaaaatttatttattaagatgcaaaagaaaggcagtgaaattgtatcacacaaattgggattcaccatgtggtgttaccacttctttcctc
ctgccctccaggtaacaagagtccctaggacttatggatcgacccaaatagagagtcacagcacccatcgcaaatataaaatatattacaataaatatat
acatctggatcataacgacctagaatgcaagtcttctgatcattagcagcagtcctgagtcattgcctacaggctttctttccaccagtctcatgtgatg
tccctttccctactctcagggagagtatggtagatccttcccacgctgttctcacatgcttttcttgccctggcatcattgctgcccttcaagtgtttct
tcttcagtttcttcatattcctctatatcatcttcctcatcttcatcacagtttggagtgaagtcaatgcttctcagacactggctgacatgttccatat
ccagggtgaactggtccatattttcaaatccatgatcagtcttctccaattggcagcctttggatgcttccattagctttttaataagctgtttggactc
cagaagaaagttagcaggtccaggctgttccatagcctgaaaagcattctgtagcacattggatgaggactggagctgttcctgataccgtacaatgaga
gaacgcacaaagttcaacttctcttcctcagcttgtgaaatccgctgtaccagatgagtctttttctcctccaggttcctgaaaagtgtctcaaacatct
ctttcacagactcctttttactctgactgntctcctgtatagttttgcatga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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