XB-CLONE-3056775
Clone Name: IMAGE:7848020
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Gene:
nek9
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168588 [+]
gtgacttcgccatgtccgccctcggccggtatgaccggcactgcgactccatcaactcggactttggcgattcggtgcgaagctgcggacctgagcagga
ggagctgcactacatccccatccgggtcctggggcatggggcttatggggaggccactttgtaccggcgcactgaggatgattcccttgttgtttggaaa
gaagttggccttgcccggctctctgagaaggaacgtcgtgatgctctaaatgaaattgtcatcttatctctgttgcaacatgataatattattgcatatt
acaaccatttcctggacagcaacaccttgcttatcgagctggaatactgcaatggtggtaacctgtttgacaaaattgtccgtcagaaggcccagctttt
ccaagaagagatggttctttggtatctgttccagattgtgtcagccgtgagctgtatccacagagcagggatactacacagagatattaaaacactgaac
atctttctgaccaaggcaaatctaataaaacttggagattatggcttggccaaacagctcagctctgaatattccatggctgaaacgtgtgttggcactt
tatattacatgtctcctgagctatgtcaaggcgtgaaatacagtttcaaatcagacatctgggcagtgggatgtgttctttacgagcttctgactctgac
tcgtacatttgatgccacgaaccctttgaatctgtgtgtaaagatagtgcaaggaaactgggcaatgggcctggacaacagtgtgtactcccaagatttg
atcgaagtggtgcatgcttgcctggaacaggacccagagaagcgcccaacagcagatgagattctgaaaatgccaatattgagctggaggagaagagaca
tggaggaaaaagtatctatgctgaatcgatccaataagaagcccaggacgggtacagttacagaagcccctatcgctgtcgttacatcaaggagcagtga
ggtgtatgtatggggaggtggaaagaccactcctcagaagctggatgttttcaagggtggatgccgagcacggcaggtctgtgctggagatgctcatttt
gctgtagtgaccgtggaaaaagagctttacacttgggtgaacatgcagggaggatcaaaactacatggacagttgggacacggagaccgtgcctcctacc
gtcaaccaaaacatgtggaaaaacttcaaggaaagtcagtacagcaagtcagttgtggaagtgatttcaccgtatgcatttcagatgagggacaactata
ttcatttggctcagattattatggctgtcttggtgtaaatcagtctgcaggtgctgaagtccttgagccgctcttggtggatttctttttaaatgagcca
gtagagcaggtctcctgtggggatagccacattattgcactgacacgaagcaagtgtgtttactcctggggttgtggggagtatggacgcttaggattgg
actcagaagatgacgtctattctcctcagaaggtagaggtgcagcgagacctctgtattgtgaatgtttgttgtggttcagatggaagtttcctattgac
tctcacaggtaaagtccttgcctgtggccttaatgagcacaataagctgggtctgaaccagtatacagcagggataattaaccatgaagccttccaagag
gtcccatacacaacctccctgacacttgccaaacagttgtctttttataagattcgcagtatttcccctggaaggactcacacagcagccattgacgagc
gtgggcgacttttgacttttggttcaaataagtgtggacaactgggagtaggagattaccggaagcatcttggaatcaatcttcttggaggacctttagg
tggcaaacaagtaattagagtgtcttgcggggatgagtttacaattgcagctacagctgataatcacatctttgcttggggaaatggtggaaatggacgc
ctagcaatgaccccaaatgaacgcccacaaggctctgatatctgcacctcatggccccgtccgatatttgggtccctacatcatgtcactgacctctcct
gtcgtggctggcacacaatcctgattgtagagaaagtactgaactccaaaacaattcgttcaaatagcagtggattatccattgggaccctcgcacaaag
ctgctcatcgggttctggaagtagagaggaggatagtgaacgtgaatctctgacctccgatcccagtagaggatttcgtggtaccatagaagctgagcct
gaaacaggtccatttaatacaactgaaaacatggaaagcagctcatgccccagctggctccgacaggagctggaggaagcagagttcattccaatgccag
atacaccaaactttgttagcatggagtcctcacaaaatgggaccacatcattgcaggatgtgaaagagactcctaatgctctagagaagccttcatttca
agcttgcacatgcagcactctacaggaggaggtgcgaaagctgcaagacgttgtgtccacctataggagtgagcaagaattactgtgtagggagaactca
cgtttggcactggaagtccaggaactaaatgccaagctccagtcagccatgcagttggccttgtattggaagagttctcctccatctgcgggcaatgctg
agacctccagggaggattctgatgcagaatcctggtgcttcttgggtacagaagcctgtcgttcttcctctgggacaccaatgtgatactccatggaagc
ctccttttgacagtctactcacacacaattaaatgtgtttaatcatatagccatgtgagcagcatgtggattctttcaggtcgatgaagagctgtttctg
tgccttaaggttaagccatgccccgctagcacactccacactggaggatactacattttttttaaattctacttcagcatttgtctctcttaacttttaa
gtgaatgaaagtttttttgcagcaaaggaacggagaaaaaaagctgtttatggtgagagtgaagggtaacatgaagtttataatgaagccactgtaaatg
gtcagcacagtgctagtgacaaaccccctcattgtgcacaattctccgctcagcttgtggacactgcataactttatttttcaccacagatgttttagct
ctctgtattgcgcgtggtcttataatgactggaatacacagatcactgtgcatggccccatattaagatcaatgttttcttattttgttctccagtcact
ttcagcgtataatcagtattggactcacaacctgtagcaagggtggagagggagacatctttggtctgtaaaccttaacacagtattgtttgtggagtac
cggagcaggcagctcgatcttgttgtctgtctcagatggtcaacttttatgccagttatgtgaagaaaaactgagctttctgtgatcagactaatcccag
caaaacagttatcagaatacattcttttaggtcagcaaaataagtggcaatggaggaatgaatgagtagaaggtgtttacaaagagaagctctgctaggt
aattgcaaatgatgaaagaaaatcctagagacagtatgtttgctgcaaggagacctgttacagagagagaagttatatgaggcaaaagcaaaggtgtatg
tgggatttgagtgataggaatgcttagtggaggttgtgcttgcacagcaagcagaggtaaacgaaaggtaaaaattattgctgctgttttttttagttaa
gggtttaaagtcaacaggaagaaatacaaaacatttacccagtgtttaagggacagcaccacttatatcgctactctaaaaaaagcatgtttgtacactg
tcaaatatatgtaaacttttaaaatgccatttgttggcatatgtgtttttgcctaaggcatggaccaccaattttgctgactaattaaacttggtcatat
gtgctcaataaaaaaaaaaaaaaaaaa
5' EST: DT532693 [+]gacttcgccatgtccgccctcggccggtatgaccggcactgcgactccatcaactcggactttggcgattcggtgcgaagctgcggacctgagcaggagg
agctgcactacatccccatccgggtcctggggcatggggcttatggggaggccactttgtaccggcgcactgaggatgattcccttgttgtttggaaaga
agttggccttgcccggctctctgagaaggaacgtcgtgatgctctaaatgaaattgtcatcttatctctgttgcaacatgataatattattgcatattac
aaccatttcctggacagcaacaccttgcttatcgagctggaatactgcaatggtggtaacctgtttgacaaaattgtccgtcagaaggcccagcttttcc
aagaagagatggttctttggtatctgttccagattgtgtcagccgtgagctgtatccacagagcagggatactacacagagatattaaaacactgaacat
ctttctgaccaaggcaaatctaataaaacttggagattatggcttggccaaacagctcagctctgaatattccatggctgaaacgtgtgttggcacttta
tattacatgtctcctgagctatgtcaaggcgtgaaatacagtttcaaatcagacatctgggcagtgggatgtgttctttacgagcttctgactctgactc
gtacatttgatgccacgaaccctttgaatctgtgtgtaaagatagtgcaaggaaactgggcaatgggcctggacaacagtgtgtactcccaagatttgat
cgaagtggtgcatgcttgcctgggacaggaccc
3' EST: DT532692 [+]attgagcacatatgaccaagtttaattagtcagcaaaattggtggtccatgccttaggcaaaaacacatatgccaacaaatggcattttaaaagtttaca
tatatttgacagtgtacaaacatgctttttttagagtagcgatataagtggtgctgtcccttaaacactgggtaaatgttttgtatttcttcctgttgac
tttaaacccttaactaaaaaaaacagcagcaataatttttacctttcgtttacctctgcttgctgtgcaagcacaacctccactaagcattcctatcact
caaatcccacatacacctttgcttttgcctcatataacttctctctctgtaacaggtctccttgcagcaaacatactgtctctaggattttctttcatca
tttgcaattacctagcagagcttctctttgtaaacaccttctactcattcattcctccattgccacttattttgctgacctaaaagaatgtattctgata
actgttttgctgggattagtctgatcacagaaagctcagtttttcttcacataactggcataaaagttgaccatctgagacagacaacaagatcgagctg
cctgctccggtactccacaaacaatactgtgttaaggtttacagaccaaagatgtctccctctccacccttgctacaggttgtgagtccaatactgatta
tacgctgaaagtgactggagaacaaaataagaaaacattgatcttaatatggggccatgcacagtgatctgtgtattccagtcattataagaccacgcgc
aatacagagagctaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: