XB-CLONE-3056372
Clone Name: IMAGE:7848153
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Gene:
mtmr9
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT532960 [+]
cctcagtggaagctattggatatttgagatttccggcaagtttaggggaagctttgcacatatcagggtctttaagcattactgtgccccccaaccctgc
catacacagtcagtgtgtaacatacagggaggggacacgtttacacacacacgtgacgcatgcactacatactcctttggtgccatcagtgtgatcctat
tagatacctgtgctgcaaatccaatggcagccaacaggtgtagataagaaatccctgcctatataagccctatagaatcagtttaatccatttccttggt
gtttgattatttaggtctagtttgccttgtgcagtgatcgtcagtctgtggctccccagaactacaactctcagtaggagagccacgggttacatagccc
taggggcctgtttataaaaactgcagtccagaataaaccctggaaaaacagatggagaaattgttgttaaatattttcctgcatttacatttccttgcag
ttttagaaataggcccctgggacatgtatatgttgttctggggttcggccaccagatggcaatgttacataatgaacacttgaatttgcctctgaaaccc
cctacctctccctcagacactcctacaggctcccaaattgcacattttgcacaacacccaaatgatctaattgcctgcacttcgttgtacctgtgtttgt
cttttcttttctcgtatatgtgattgtaattaatagattatttgtgaatgcttaaactaaccttaggggatgtcacacttctgacctgccaaactgcagt
ttccgacggctggctgagcatgctgggagctgtggtcctcggcagcagcagtagaggtgatngtatgttctatctctat
3' EST: DT532959 [+]tgaagtctacttcattactgtacaaacattctcagtcacaaatacattagctttccctttgatatttaatacacggatagagatagaacataacatcacc
tctactgctgctgccgaggaccacagctcccagcatgctcagccagccgtcggaaactgcagtttggcaggtcagaagtgtgacatcccctaaggttagt
ttaagcattcacaaataatctattaattacaatcacatatacgagaaaagaaaagacaaacacaggtacaacgaagtgcaggcaattagatcatttgggt
gttgtgcaaaatgtgcaatttgggagcctgtaggagtgtctgagggagaggtagggggtttcagaggcaaattcaagtgttcattatgtaacattgccat
ctggtggccgaaccccagaacaacatatacatgtcccaggggcctatttctaaaactgcaaggaaatgtaaatgcaggaaaatatttaacaacaatttct
ccatctgtttttccagggtttattctggactgcagtttttataaacaggcccctagggctatgtaacccgtggctctcctactgagagttgtagttctgg
ggagccacagactgacgatcactgcacaaggcaaactagacctaaataatcaaacaccaaggaaatggattaaactgattctatagggcttatataggca
gggatttcttatctacacctgttggctgccattggatttgcagcacaggtatctaataggatcacactgatggcaccaaaggagtatgtagtgcatgcgt
cacgtgtgtgtgtaaacgtgtcccctccctgtatgttacacactgactgtgtatggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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