XB-CLONE-3056310
Clone Name: IMAGE:7848159
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Gene:
psmb7
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533001 [+]
tttcagcaactgcaggagaaataccctcttgaccaatgaatttactcagaaagggcacaagctacccgcagctcgcaaaacagggaccacaattgctgga
atagtgtttaaggatggagttatacttggagctgacacaagagcaacagaaggaatgattgttgctgataagaactgctcaaagatccactatatagctc
caaatgtctattgctgtggtgctgggactgcagctgatacagaaatgacaacgcagatgatctcttccaacatggagctacattctctgtccacgggacg
tttatccagggtagcaactgccaaccgaatgctgaagcagatgctatttaggtatcaaggttatattggtgctgcgctggtactgggtggagtggactgt
actgggccccatctgtacagcatttaccctcatggatccactgacaagcttccctacgttactatgggctctggttcgctggctgccatggctgtttttg
aggatcgctacaaacctgacatggaggaagaagaagccaagcagctggtacgcgatgccatcgccgctgggatctttaatgacctgggctccggcagcaa
cattgatctatgcgtaataacaaaaaataaggtggattatatccgtccccacgagttggccaacaagaaaggcgtgaggacaggaaattataaatacaag
aaaggtaccacggggattctgtcggagaagattaccaacttcaacttggatgtgatggaagaatccgtgcaaactatggatatttcctaagtctcttaag
taaatgtattgncatctttttgcatcccgtgttagcccacattatcatgttaagcattttctgcgttatcttatgatcca
3' EST: DT533000 [+]ttttaagttttgttttttttaatggatcataagataacgcagaaaatgcttaacatgataatgtgggctaacacgggatgcaaaaagatgccaatacatt
tacttaagagacttaggaaatatccatagtttgcacggattcttccatcacatccaagttgaagttggtaatcttctccgacagaatccccgtggtacct
ttcttgtatttataatttcctgtcctcacgcctttcttgttggccaactcgtggggacggatataatccaccttattttttgttattacgcatagatcaa
tgttgctgccggagcccaggtcattaaagatcccagcggcgatggcatcgcgtaccagctgcttggcttcttcttcctccatgtcaggtttgtagcgatc
ctcaaaaacagccatggcagccagcgaaccagagcccatagtaacgtagggaagcttgtcagtggatccatgagggtaaatgctgtacagatggggccca
gtacagtccactccacccagtaccagcgcagcaccaatataaccttgatacctaaatagcatctgcttcagcattcggttggcagttgctaccctggata
aacgtcccgtggacagagaatgtagctccatgttggaagagatcatctgcgttgtcatttctgtatcagctgcagtcccagcaccacagcaatagacatt
tggagctatatagtggatctttgagcagttcttatcagcaacaatcattccttctgttgctcttgtgtcagctccaagtataactccatccttaaacact
attccagcaattgtggtccctgttttgcgagctgcgggtagcttgtgccctttctgagtaaattcattggtcaagagggtattt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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