XB-CLONE-3056268
Clone Name: IMAGE:7848115
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Gene:
hhatl
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533029 [+]
acttataatagtaaaatatttattaaagctactttccctgtggcacatcggttcctatacagacaaatgtaagggctggtggcagattgtttggaacctg
cctgccctccggtgccggctgaactacaactctcagcttcccgtgtagctaagcgctgatatccccagcacagaggacgctgctcttttgcgtttcattg
gtttctacgaacaaacgcggcgactaggcgactttctgcttctcaagcctctcttgctcgatggccattagccgctctcgctcctttatcagctgcaccc
cgcagtacgtaacaagccagatggacagggtgctttctgggaaacctgtaattagaagcctcttggctaccagcagagcaaagtccaggctgtttagggc
caatatgttatagaaaataattgcccagaagttggctgcaccgaaaacccctctgattctcctggacatggactctggcaacatggcctcaaatcttgca
aagggtcccagctggaagaatttctgcacccagagttcaaagttcaggccgaagcagttgcagacggaccagatataaacgatttcacacgggccgagcc
ataaagtagtgacgaggaaagtagccacggtggctattaactccctctttatgttatcgtggccttccccgatgtagtcatagacgtatttgcagagcca
gtcattgatcccacggtcgaagtgcgtttccgcgaagacataaagcatggtgatacacttgggcggctgtggggggtcgaggtggtccagcctggagatg
gtgtttataactccgaacatcacggcggctttcacccagtcgtacacca
3' EST: DT533028 [+]attagcctaaattctcccaatatcgcccaccctctagtgggggcctccagccagacagcctggccataaagcatcacctaaaccatttctacaaaaactg
catactcatgatttctgtaaatgttttccttcccctttagaattggatctctgagccgtctgtgccgctaaagccgcaccatgggaatcaaaacggcgct
gccgcactacgagctgggcttctatgccctcgtggtgacttgtgccttggtctactccctcaatggaataatcgaagcctccagggatggaatgaatcgg
aagtcatttcgggagaacataaaggagggctggcattacttcgggaggaaaatggacgttgccgacttcgaatgggccatgtggttcaacacattcagga
actacattatctttgccctttcgggacacgtcatctttgccaagttcttttcgctaatggttcctcagtacagatcatacgtttacatgctgtacgggat
cctgacggtactggcgatcatgggaaccaactacctgatgctcatcttgtcccactgtttcttcctctacaccatttcgctggttaaaagcaagtggctg
tgctttgctgcaggcctctgtagcctggccaccttcaaaatggaaccgttcaacacttggcaaagtggctttgtcacggggacgtttagcctccaagaca
ttctcttttacggaggcagtggattttccatattgcgctgcatgagcttcgctctggagacctggagaggaaacaagggttctactccccgatggaactt
ctcnaatacaatttctacctccctttctttttctttggccctattatgacttttgaccagtttcacgtgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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