XB-CLONE-3056221
Clone Name: IMAGE:7848215
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Gene:
ttc7b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533060 [+]
tcgatctccgtctccaggcgggaaccagcctttttggtagccatggctagctagacagcgaagctaactggagaaggtgggcggggaggcctgagcacag
gccccggctgagtagcacaagctaccggccggcaaagagtcactgacaggagagtggatataagagcggcgccaggctgtcacacagggaactacacacc
cacacagctcactccatggggttttgtagtttcctccccccctattctttatagatatctttaatagaaaatgtaacccaaaaatgtttgcctaaagaaa
gaaactgtaactttaagcaacgttgcaatataaatgcatgatacatttatagtaatgttactgctatttgtgaagtaaatctgctactgcaaacagtata
tccctgcagcgttctgttctcagtcctggtggtgctgccttctgagagaatttaacagaagcccgctgattagcagatataaaaggtagacttcgctact
tcggtttaagagacagaactagcagtgcagaaatggacagacatatacagggccggatttgtcttctgggctgccttggggcgccccaccctctccagtg
cttaaacgggacattattgagttagagagggtccagagaagggcaactaagctggtaaagggtatggaaagtctcagttatgaagaaagactggccaagt
tgggtctgtttacactggagaagaggcgcttaagaggtgacatgatagctatgtataaatatataaggggatcatataataacctctctaatgctttatt
taccagtaggtctttccaatggacacgagggcacccgct
3' EST: DT533059 [+]gcagctggattacagcatataaaaatgtagtttattcatactttttgaaggatcagattacagtgataggtatattaggggtttctgtgttagtaaatag
taagttaggttgaaaaaagacgtacgtccatcacgttcaaccataatgcctatatataacctgcctaacttctagttgatccagaggaaggcaaaaaacc
ccatctgaagcctctttaattccttcctgactccaagatggcaatcggacctctcacagtaaaaaatcctttccgaatatttaaatggaaccttccttct
tctaaacggagcgggtgccctcgtgtccattggaaagacctactggtaaataaagcattagagaggttattatatgatccccttatatatttatacatag
ctatcatgtcacctcttaagcgcctcttctccagtgtaaacagacccaacttggccagtctttcttcataactgagactttccataccctttaccagctt
agttgcccttctctggaccctctctaactcaataatgtcccgtttaagcactggagagggtggggcgccccaaggcagcccagaagacaaatccggccct
gtatatgtctgtccatttctgcactgctagttctgtctcttaaaccgaagtagcgaagtctaccttttatatctgctaatcagcgggcttctgttaaatt
ctctcagaaggcagcaccaccaggactgagaacagaacgctgcagggatatactgtttgcagtagcagatttacttcacaaatagcagtaacattactat
aaatgtatcatgcatttatattg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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