XB-CLONE-3056133
Clone Name: IMAGE:7848176
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Gene:
snta1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC124016 [+]
CCCCTCTCTCCCCTCCCTCTATCCCTGTCCGGGCACTCTGGCCATGGCTTCCCCGGGCACGGCTCACCGGAGCGGGCTGCTGGAGCTGCGCTGTCCCGGG
GAGCGCTGGGTGCGGGTACTGCTGCTGCTCGGAGAGGAGACTGTGACTTTCACCCCGGAGCCGGAGCCCGGGGCAGCGGAGGAGAAGGCGCTGAACGGCG
CGGAGCCGGCGGGGGGGGTCCCGGAGTCTCTCATTAACCTGCGCCGGACTGTCAGGGTGCTCAAGCAGGATGCGGGGGGGCTGGGGGTCAGCATCAAGGG
TGGCCGGGAGAACAAGATGCCCATCCTCATCTCCAAGATTTTCAAAGGCCTGGCTGCTGACTTGACAGGGTCATTATATGTGGGGGATGCAATCCTGTCT
GTTAATGGGGTAGACCTGTCAGAGGCTACTCATGATGACGCTGTGCAGGCTCTGAAGAAGACTGGAAAGGAGGTGGTGCTGGAAGTAAAGTACATGAAGG
AAATCTCACCGTATTTCAAGGGCTCTCCCCCGAGTGGTTCACCTGCTGCCCCTTTACAGAAGAGGGGGTCCCCCGCGCTCCCACCCAAGGAATACAAAGA
TGGAAAGAGCATTCCGCTGAAGATGTGCTACGTATCCCGGAGATTCCTACCAACGGACACCGAGCCCAGGTACCTCGAGATCTGCTCAGCAGACGGAAGG
GATGTGCTGTTCTTTCGGGCAAAGGATGAAGGTACAGCCCAGTCCTGGTTTAATGCCATCCACACCAATGCCAGCGCCCTGGCGCCCCGTGTGCGGGAGG
ATGTGAAGCAGCTATTGGGCAAAGACATCAAACAGATGGGCTGGCTCACTGAGCAGCTCCCCCAGGATGGAAAGCGCAATCTGCTGGCCCTACTCACAGA
GAAGGATCTCCTGCTGTACAACAGCCTCCCGCACTCCAGGGAGGGCTTCAGCAAGCCTGCTTATTGTCACCCACTCATTGCCACCAGGTTGGTACACTCT
GGGCCCTCAAAGAGCTCTCCTCTCTACGACACAGAGCTGGCATTTGCTTTGCGTTCTGGTACCCAGAAAGGGGTGGAGACTCATCTGTTCAGCGTGGAGA
CGCAGAGGGAACTGGCAACCTGGACACGGGTACTGGTGGACGGCTGCCACAGTGCAGCAGAACTCATAAAGGAGGTGACCACAGCATGTACATGGGGTGG
GAATGAGTGTGCCCTGTCTATACACATTGATCAAGGTTTCACTCTCTTCACGGAGGAACCCGGCCTTAACAAGGTGATACACTTCCACCAGCCATTCGAG
AAGCTCCGTATGTCGTCTGATGATGGTGTCAGAATTCTCTATCTGGAATTCGGCAACCCCGAGGCAGAACTTCAACTCGACCTGCACTCCTGTCCAAAAA
CCATTGTCTTCATCATTCACTCCTTCCTGTCGGCCAAAGTGTCCCGACTGGGCCTATTCACCTAGAACGCTGAAAGTGGCGGCAATCTTGGACCTCGCGG
CCGAGCGCTAACCAAAACCAACCCTAGGGGGTGCTGGAGAGTTAGTCCAAAGTGGACAGCTTTCCAAAATGTCACACAAATAATATTTGTATTTACACAC
CATAAATGGCCAGAATTAACCACTTCTATGTCACTATGTCTGGATACGGTTAATGGCAAGGAAATGTGAAGCCTCTCGTCTTTATTCTTTGTAAACTGCC
GATTCCCTAACCATGAATTATAGTGCCTTATATATATATATATTTCTTTTTCAAAGGTTTAATGCCATGGCTTCAGGGAGAATCCAGGTCAGTCTCTCTG
ATATACAGTGGGTTTTTGGGGAAAAACAAAAGTACTGTGCCTTTAAATGCCCAATATCTGCTCCACGCCATGTTGTTATAAAGCAGCACAACCCGCTCTA
CAATCAGCTATCAATCTGGTCTAATTGTTTCCCCTTCGGAGCACAGGTTAGGGCGTTTGTATAAATATATTAATATATATTCATAAATGATCATAGAGGC
AGATATGTATCTAGTTTTATTAATTATTGAAATTAAGCTACCCGGCACAACTTTTTAAGCTGCATTAGAATAAGGGCATTTGGGGGGGTTTATTCTGGAC
TTGAACCTATTATTTTACAAAGTATTTAGCGTGTGGATATAGGTATATATATATATGGGAGGACGTTTCATGATCCTGATTGTTTGGAAAGAAACGGAGG
TGTTTACTTGCCCTTTGGAGGTGTACAATAAACAATAAAGTGTCTGAGCAAAAAAAAAAAAAAAAAA
5' EST: DT533118 [+]cccctctctcccctccctctatccctgtccgggcactctggccatggcttccccgggcacggctcaccggagcgggctgctggagctgcgctgtcccggg
gagcgctgggtgcgggtactgctgctgctcggagaggagactgtgactttcaccccggagccggagcccggggcagcggaggagaaggcgctgaacggcg
cggagccggcggggggggtcccggagtctctcattaacctgcgccggactgtcagggtgctcaagcaggatgcgggggggctgggggtcagcatcaaggg
tggccgggagaacaagatgcccatcctcatctccaagattttcaaaggcctggctgctgacttgacagggtcattatatgtgggggatgcaatcctgtct
gttaatggggtagacctgtcagaggctactcatgatgacgctgtgcaggctctgaagaagactggaaaggaggtggtgctggaagtaaagtacatgaagg
aaatctcaccgtatttcaagggctctcccccgagtggttcacctgctgcccctttacagaagagggggtcccccgcgctcccacccaaggaatacaaaga
tggaaagagcattccgctgaagatgtgctacgtatcccggagattcctaccaacggacaccgagcccaggtacctcgagatctgctcagcagacggaagg
gatgtgctgttctttcgggcaaaggatgaaggtacagcccagtcctggtttaatgccatccacaccaatgccagcgccctggcgccccgtgtgcgggagg
atgtgaagcagctattgggcaaagacatcaaacagatgggctggctcactgagcagctcccccaggatggaaagcgcaatctgctggncctactcacaga
3' EST: DT533117 [+]gctcagacactttattgtttattgtacacctccaaagggcaagtaaacaccctccgtttctttccaaacaatcaggatcatgaaacgtcctcccatatat
atatatacctatatccacacgctaaatactttgtaaaataataggttcaagtccagaataaacccccccaaatgcccttattctaatgcagcttaaaaag
ttgtgccgggtagcttaatttcaataattaataaaactagatacatatctgcctctatgatcatttatgaatatatattaatatatttatacaaacgccc
taacctgtgctccgaaggggaaacaattagaccagattgatagctgattgtagagcgggttgtgctgctttataacaacatggcgtggagcagatattgg
gcatttaaaggcacagtacttttgtttttccccaaaaacccactgtatatcagagagactgacctggattctccctgaagccatggcattaaacctttga
aaaagaaatatatatatatataaggcactataattcatggttagggaatcggcagtttacaaagaataaagacgagaggcttcacatttccttgccatta
accgtatccagacatagtgacatagaagtggttaattctggccatttatggtgtgtaaatacaaatattatttgtgtgacattttggaaagctgtccact
ttggactaactctccagcaccccctagggttggttttggttagcgctcggccgcgaggtccaagattgccgccactttcagcgttctaggtgaataggcc
cagtcgggacactttggccgacaggaaggagtgaatgatgaagacaatggtttttggacaggagtgcaggtcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: