XB-CLONE-3056058
Clone Name: IMAGE:7848136
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533168 [+]
ctccagaagtcgagtggtttaaagacaatatacctgtttctttgtcctcccatataagagtttcacgttcaaaacaggtctacactcttgaaataatcca
cgcaacagagaaagatattggaaaatacacaattaaagcaaagaacttccatggacaatgctctgccacagcatccttaaatgtgcatcatcttatggaa
gaacctgtcaagcaggttgtattacagacagctggggaagtcagccttcaggaaggattctccttccaggcaatgaaaatgtcttcttccaggcaagagg
cctccttcagcagtatctccgagacaaagagcatgtcctcaaaaagtgaggcatcatttgttgaaatgagttcaagtagctttatggaaaaatccagtct
gatgcagagttcatctagcagaatgcttacagctggtttcagaggaatacctcccaaaattgaagctcttcccaatgatgtcagcattgacgaaggaaaa
gtacttactgtggcttgtgccttttctggagaacccacaccagatattgcatggtctaaggctggacatccagttcccactcaagaacaaaaaggcagag
ttcacattgaaacttctgaagacttaaccactcttatcatcatggacattcagaagaaagatggcggacattacactcttaacctgtcaaacgacttcgg
gtccgattctgcatctgtgaatattaatgtccgaacacattaagcattttatcgtttacattcacatgtaattttacaagtgattcactagaactagatg
aactgatttgtaaatataaaagctatttttgtacctaccctgtatagacaaagtcg
3' EST: DT533167 [+]tttttttatggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaata
aataatgacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagt
ccctattctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggc
caggtgatgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaaca
tacaaaagttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacct
aatattcacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaa
cctgctgaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgtt
ggttccagtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgt
atcaatttccctattcaaataggaatatgtgatagcgtaaatcttttcgactttgtcttatacaggtaggtacaaaaatag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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