XB-CLONE-3055823
Clone Name: IMAGE:7848252
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Gene:
farp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533324 [+]
ctcctcctcctccagccattctaggctcctgtgaatcctgttcgcaaataggctgtgccgtttcgacaatggcgaccctgaaacagatgcccttccacac
ggcagcctgaactcataccttctaatggaagtgcggccgcagcatcactagtgcagaagcagccgaggaccgagctacagtgcagaaggaggagtgaatt
gccaagccggggatcttatacagtgcaaaagtaagaggaactggatttcttgagcatgggggaatcggagcagagacaaacggccgcttctcgattagga
gctcccgaaagtgttggtatcagcacactagaacatggagtcaagccgcctttatcaccgcaggggaaaatctatccattcaaggtccagatgctggatg
aaaccttggagaccgttgaagttccaataagagccccaggaaaagctcttttggatgctgtctgtagccaccttaatcttgttgaaggggactattttgg
ccttgaattccaggaccatagaaagattatggtctggttggatctcttgaagccaattgtcaaacagattagaaggcctaagaatgtggtgcttaagttt
gtagtcaagttctttccacctgatcacactcagctgcaagaagaattaacaaggtacctctttgccctccaagtcaagcaggatctagtacaaggaaagc
ttacttgtaacgaaagcagtgctgcacttcttatctctcacattgtacagtctgaaattggagattttgatgaagctggggaccaggaccatttgcaaaa
gaacaagtatatgccccaacaagaacctttggaagaaaagatcatggaattacatcggaatcattttggccagacaccggcagagtca
3' EST: DT533323 [+]gcttgtgcatttcatttattgcatcttggtaaaatcccgcccttcctggtttagccggtgcatttatagtgcagtggggcagtgtactaattaaaagaag
ttctaaagcacaaaaaaagaatatatatatttataaaaaataaaaaggcacaacatccatgtcaaaaaagtccggagaacaatcggatgcaaagtttgta
cattaaaaaaaaaaatttcattttccaaagatgcctgtgcactgatagtgaggaactgcatggcgtcagttaattctgtggttctggccatatgggaaag
ctgtggctgtataaagggagaggagttttagtaaatacaggttagagctagcggtgtttcttgcttccaaactgagctgattaaagtgctttgtaggcca
gtaaaccttcatggaaacaactctatagcaggacaattgaagggaaagctttataatttaaacacttgaaaaacattgaccttttgttccaatgatacac
ggacaagttaaactgcaagtgggagttagaaacaggacagggatacaagtagaatcattggccactagactagagacaagatttttgttagtgttggcac
atgttcaattcagagtttatagtatagagaaggcctggctcacagaatggttttaaaaagtttaaaaatgtgtttgcccatacgatgctacaaaaagaaa
ggcattgttgggcaggatccttgggtttctgtgaaacaacagctgccggcctcacaaaaattcagcaagagctggggctggtgtcggattcccgctggca
aagcagccggcccatttagtgtcaggaatgttcagtacatgtgggactccttgcggctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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