XB-CLONE-3055606
Clone Name: IMAGE:7848511
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533467 [+]
aaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcagaggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggagaga
agacagaagggaatgagagtgcacctgaaggagaggggggttcaaatcagcaacgtccataccgcaggagacgtgttccaccatactacttgcggagacc
atatgggcgcagaccacaatactcaaatgctcctattcagggagaaggtgcagagggatcagatggtcaaggttcttcacaaggtgatcaaggtagacct
gtgcgacagaatatgtacagaggcttcagaccacgatttcgcaggggaccaccacggcagagacaacccagagaagaaggaaatgaagaggacaaagaaa
atcagggggatgaaacccagagtcagcaggcacctcaacgtcggtatcgccgtaactttaactacagacgcagacgcccagaaaaccctaaaccacaaga
tggtaaagagaccaaggcagccgaaacatcagctgagaacacgtccgctcccgaggctgagcagggcggggctgagtaaataccggcttaccaactctac
caccatccggttttttgtcatcaaaatgtcaagacagaaattccagcaataagaaatgaacaaagaattggaactgaagaccttaagtgcttgctttttg
ctcttgaccagataacttcatcctgcatgggtaaatgcagagatggtttttatgtttttttttctaaaagctccttttgggaatgaaaattttttaaaaa
catattttttctncatgtacctttcaaaggtttttaaattgtttcctatctggtcaaactaagattttttaagaacctcatttt
3' EST: DT533466 [+]tttatgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaaa
tcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagctttta
gaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaattc
tttgttcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccgcc
ctgctcagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtctg
tagttaaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctgggtt
gtctctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgacc
atctgatccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcctg
cggtatggacgttgctgatttgaacccccctctccttcaggtgcactctcattcccttctgtctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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