XB-CLONE-3055488
Clone Name: IMAGE:7848617
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Gene:
gapdh
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533545 [+]
catcgattcggtgacaccaaaatgactgtcaagattggaatcaatggatttggccgcatcgggcgcctggtgacccgcgctgctgttttaagcggcaaat
gtgaagtcgttgcaatcaatgaccccttcattgacttggactacatggcttacatgttcaaatatgactccacccacggccgattcaagggaacagtgtg
tgttgagaatggcaagctggttatcaatggcaaggcagtcaccgtcttccaagagcgtgacccctccaacattaagtggggcgatgctggtgcagtgtat
gtggtggaatctactggagtcttcacaaccaaagagaaggccggtttgcacttgaagggaggtgccaagcgtgtcattatctctgccccctcagctgatg
cccccatgtttgttgttggggtgaaccatgacaaatatgataactctcttcacgttgtcagcaacgcatcctgcacaaccaactgcctggctcctcttgc
aaaggtcatcaatgacaactttggcatcctggagggactgatgactaccgtccatgccttcactgccacccagaagactgtggatggcccatctggcaag
ctgtggagagatggcagaggtgccggtcagaacatcatccctgcctcaactggtgcagcaaaggctgttgggaaagtcatccctgagctgaatggcaaac
tgacgggaatggctttccgtgtccccaccccaaatgtctccgttgtggatctgacctgccgtctgtccaagcctgccaagtatgatgacatcaaggctgc
cattaagactgcagcacatggcccaatgaagggaatcctggaatacacagaagaacaggttgtctccactgacttc
3' EST: DT533544 [+]gatgtttcattgttttatttagtaacacagaaacagtgacacagacaaactaccaggatgggatcgtgactctgggccccttcagtgacagagaggggtt
ggtggggcgacaggtaagtgcttattccttagaggccatgtgacacatgagatccacaacacggtggctgtagccacactcgttatcataccaggaaacc
agcttcacaaagttatcgttcagtgcaattccagctccagcatcaaagatggaggaatgagtgtcaccattgaagtcagtggagacaacctggtcttctg
tgtattccaggattcccttcattgggccatgtgctgcagtcttaatggcagccttgatgtcatcatacttggcaggcttggacagacggcaggtcagatc
cacaacggagacatttggggtggggacacggaaagccattcccgtcagtttgccattcagctcagggatgactttcccaacagcctttgctgcaccagtt
gaggcagggatgatgttctgaccggcacctctgccatctctccacagcttgccagatgggccatccacagtcttctgggtggcagtgaaggcatggacgg
tagtcatcagtccctccaggatgccaaagttgtcattgatgacctttgcaagaggagccaggcagttggttgtgcaggatgcgttgctgacaacgtgaag
agagttatcatatttgtcatggttcaccccaacaacaaacatgggggcatcagctgagggggcagagataatgacacgcttggcacctccctttcagtgc
aaaccggccttctctttggttgtgaagactccagtagattccaccacatacactgcaccagcatcgccccactttatgttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: