XB-CLONE-3055431
Clone Name: IMAGE:7848765
|
Gene:
ccng1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT533583 [+]
ccgagggggaatgctcgtctcggtaaaaatgaaaccttaagctcagtataaaatatgctttaacttagcaacattttcagcggctttgtgtgtgtgtgtg
tatatgtgtgtatatgtgtgcttgtgtttttaactgtctagagcaaccgtgtttatgttcctccgtctgacttttccgttgcatcttcacgaacatggat
actgtatttatttgagtacctgggactttaaaagtacgcaagatggcctactacgatacacatatatttattcccatataataaaagtctattctgtctg
cactggaatggtcaaaagaacagaggataacacacaatataataagatttacctgattttagttttccatgggctcttttagtacatcagtgttatgtaa
cccaattaatttattcattattcatagtatacctttagaaataagttactctaggctagtactccctagcaaccaaatgggaattctgctttgattagtc
ttaaatgcagttaatttatcatggcttattgctgtttggttgctgtgagttcctactgtaaagtaaaatgacacttttctccttatactgtcctccgaat
gtgtcggtggctaaagtattgtgtatgaatagtaacaacagaaacagaagctaagcaaaggtttgcaaaactgggtataacgttgtgggtatatgggtaa
actacagcacttgcttattatcagcaggtcggtaagctggtacatgtagtaatacagaccactgtatagaaatggtgacaaaagtgcctctatacaatgt
aataagatttgtacctttcaataaatgtattgaaatgctgaa
3' EST: DT533582 [+]tttttcgcatttcaatacatttattgaaaggtacaaatcttattacattgtatagaggcacttttgtcaccatttctatacagtggtctgtattactaca
tgtaccagcttaccgacctgctgataataagcaagtgctgtagtttacccatatacccacaacgttatacccagttttgcaaacctttgcttagcttctg
tttctgttgttactattcatacacaatactttagccaccgacacattcggaggacagtataaggagaaaagtgtcattttactttacagtaggaactcac
agcaaccaaacagcaataagccatgataaattaactgcatttaagactaatcaaagcagaattcccatttggttgctagggagtactagcctagagtaac
ttatttctaaaggtatactatgaataatgaataaattaattgggttacataacactgatgtactaaaagagcccatggaaaactaaaatcaggtaaatct
tattatattgtgtgttatcctctgttcttttgaccattccagtgcagacagaatagacttttattatatgggaataaatatatgtgtatcgtagtaggcc
atcttgcgtacttttaaagtcccaggtactcaaataaatacagtatccatgttcgtgaagatgcaacggaaaagtcagacggaggaacataaacacggtt
gctctagacagtttaaaaacacaagcacacatatacacacatatacacacacacacacaaagccgctgaaaatgttgctaagttaaagcatattttatac
tgagcttaaggtttcatttttaccgagacgagcatgtcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: