XB-CLONE-3055329
Clone Name: IMAGE:7848535
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533651 [+]
cccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaatca
tatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagttgac
aacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgaggatc
gtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgagcag
ccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatcaaagctctc
agcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttgaca
aaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgggt
aaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaagag
atctttaaacagcaggacaccccttcatgtggaatgaacatctggngtatgtccttacctgcccatcanacttgggcacaggcctgagaggaggtgtcca
tgccaagctccccaacctcagcaa
3' EST: DT533650 [+]agaggtgatattttggttttattgtctccaggcaatcattggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatc
agagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatc
cattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgttt
aggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatgggctgtcccttctcaagcttcttctccatctcaatcatgagttt
cacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaaca
ccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctca
ggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctg
aagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttaccccacacagggag
gtcttgttttcattgtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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