XB-CLONE-3055148
Clone Name: IMAGE:7848695
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Gene:
loxl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT533771 [+]
ctttcgtttgggtgctgtaggataagatgaaatccagtgggactatcagctttgcttattggaaggtgcagcttgtgtaatcctgtccctttcccacttg
agcggtgcgccccccgtttccctggctctgcgtcaggctaacactggcgctaccaccaaaccactgctgctacccacttgttatattggtgctattaaac
ctggaagtatgcctacaagcacttctttggactcttcccccgttctatttccgtggctacagtcagtgatcctggctctcttctgtgcttatcatctctc
tccactagggggaagcacactggagggtacatggagtgctcagggccagctatggcagatacatgccgcctttccaacattaggttgattcattctttaa
atacaaaatacatcatgtatatcagtagggatcatctttcatttttcccttgttactgcccagtaacctgtgaagttagagcttatcactcaagcacaat
ttccttcacttaatacatcaatctgtacatgtaggggggctgccatattgtttcttttagcatactctttaaaagcaagagccctctggttcaggacaga
aaggctcactgcctgtatccataactcccaccattcctggccaacaaaccagcagaagatcataaatgtaaacctttttgtatgacagggatatctctat
ggccctttagctggtgctgaaccataattcagctgggaatgctgggagctgtagttcaatggatttccctgctgtagactattcagtgacctagatctaa
cttattctatattttttaaatgtaaagtaaagattatttttcccttatatgttatgctatttgctac
3' EST: DT533770 [+]gtgctcacgaacagttttatttccccaacaatgttatggtcacatacagggagggagaacctggaagctttcataattcaagtataaagcaggcagcata
tggggggaaatggctgctctcctccccgtgcctgtccttggttactccccctcctctgcagcagtggtacagccagctgcctcaataggcagagtcagga
ctgcaagaggcactgaaaatcattgtaataattataataagttatcatttattcctagacatatgctaagactaatactgggcggagtaacgtagcaaat
agcataacatataagggaaaaataatctttactttacatttaaaaaatatagaataagttagatctaggtcactgaatagtctacagcagggaaatccat
tgaactacagctcccagcattcccagctgaattatggttcagcaccagctaaagggccatagagatatccctgtcatacaaaaaggtttacatttatgat
cttctgctggtttgttggccaggaatggtgggagttatggatacaggcagtgagcctttctgtcctgaaccagagggctcttgcttttaaagagtatgct
aaaagaaacaatatggcagcccccctacatgtacagattgatgtattaagtgaaggaaattgtgcttgagtgataagctctaacttcacaggttactggg
cagtaacaagggaaaaatgaaagatgatccctactgatatacatgatgtattttgtatttaaagaatgaatcaacctaatgttggaaaggcggcatgtat
ctgccatagctggccctgagcactccatgtaccctccagtgtgcttccccctagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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