XB-CLONE-3054758
Clone Name: IMAGE:7848540
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534028 [+]
agaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccgatgagtccacaggcagcatcgctaagcgcttgagttc
catcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccgacgacagagtgaacccctgcattgggggcgtcatcacc
ttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtg
tcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgc
caagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaa
cagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcag
tgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatggttacccccgggcacgcctgcaccaagaagtacccccc
tcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggtcagagtgaggaagag
gccaccgtgcacctcaatgccatcaaccagtgccccctgatcaaaccctggcccctgaccttctcctacggccg
3' EST: DT534027 [+]ttttttttttgggtcgaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgt
agcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcag
tctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcact
aggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctc
tccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccc
caggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacgg
tggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctc
ctgaggggggtacttcttggtgcaggcgtgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttg
tacactgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatcanggagaacctcgggctccaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: