XB-CLONE-3054594
Clone Name: IMAGE:7848650
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534135 [+]
aagtttgctccgtctctaaagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggagga
atatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcacc
ttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttca
aggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtga
tgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgt
gccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagc
agctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggca
caatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgc
ttctgtgagggacttcagaagattgaagagatctttaaacaagcaggacaccccttcatgtgg
3' EST: DT534134 [+]tttggttttattgtctccaggcaatcatggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagat
ttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgt
attcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctc
tagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccac
taccatctggacttggtcaacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcct
ctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgccca
agtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcaca
gaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttca
ttgtgccagataccacgggcatcaggccagtcacggcccatgccagaggccagaagcagagggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: