XB-CLONE-3054573
Clone Name: IMAGE:7848939
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Gene:
vegfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534149 [+]
aatacacagaagcttctgcagcttcagaccagctgtgaccttacagtcacatgcccttgtacggaggctctgattggctgtaccccgagcattctcttgt
cccgggggtaccaacatggccgctgcctgtgccgtctctttccttccatggctttcccgcagcatcgctccagtcttccctcgggttttgccctttctct
ctctgtctctccttcttttatttttttctttcagaaaagcaaaacgagggaagggccttaaacgaaagcgcaagaaaatccggtacaaaccgaagcactt
atgtgaaaagccgcgccggtgagcgccccccctgccgacacagagtccatatgaaggatcggtactcagcacagcactttggtttcaagcattccgacaa
gagggacgggacaagcccaccagattctccttttccttcccacctttattgctgctaaacgccaggcaccccgaacccccaggcttccttcatctgttgt
ttctggagaaatagcagccaaattctgaatgtgccccccctgcccaatacggggcacacatgggcacagccggatgttttgggccgatacagaacttctc
ttactattcgatttgtttcctttggatttgcagttcagcaatatgattggaggatacggggggcaccattgggaaaccctccctgccctcggacttgcta
agagccgccatcttggaaaggttccgagaatgtttcagtgacttctagagactcgacgcccgtgtatttggactaaagctgaagcccattctctccatta
cccggaaccgcatcctcgtggctctgggtcggacctgcaggagcgcatagagacgtaaataccagactcgctatggccgccagtttgtcagggccgcgct
ca
3' EST: DT534148 [+]aaaacgtgagagcctgaagactcccccactggaaggcaagcctcactcaactcaggcaagtctcattcaaaggaaagcaggctgcactcagtatgagtga
gtcccgctcgtattacagcacttccactcattatgagtgagtcttcctcaatgaggtgagaatgaactcattgtatcagttccaccaatcgccccttgag
ttactgctttgctcctgggggtcccgtgggcactgggctgagcgcggccctgacaaactggcggccatagcgagtctggtatttacgtctctatgcgctt
cctgcagggtccgacccagagccacgaggatgcggttccgggtaatggagagaatgggcttcagctttagtccaaatacacgggcgtcgagtctctagaa
gtcactgaaacattctcggaacctttccaagatggcggctcttagcaagtccgagggcagggagggtttcccaatggtgccccccgtatcctccaatcat
attgctgaactgcaaatccaaaggaaacaaatcgaatagtaagagaagttctgtatcggcccaaaacatccggctgtgcccatgtgtgccccgtattggg
cagggggggcacattcagaatttggctgctatttctccagaaacaacagatgaaggaagcctgggggttcggggtgcctggcgtttagcagcaataaagg
tgggaaggaaaaggagaatctggtgggcttgtcccgtccctcttgtcggaatgcttgaaaccaaagtgctgtgctgagtaccgatccttcatatggactc
tgtgtcggcagggggggcgctcaccggcgcggcttttcacataagtgcttcggtttgtaccggattttcttgcgctttcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: