XB-CLONE-3054508
Clone Name: IMAGE:7848897
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Gene:
mypn
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534192 [+]
ccatcgattcgttcattcgctccttattgatcctgtcacaaaagcagattctgggacctacatttgcattgcaactaataaaactgggcaaaactcattc
agcctggagctcagtatcatggcaaaagaagtccaaaaagttccagtatttttggaaaaacttcaaaattgtggtgtcccagaaggtcatccagtcagat
tagagagtcgagttgttggaatgcccccaccagtgttctactggaagaaagacaatgaatccattccggccaataaaccacggataagcatgcatcaaga
taacactggttacgtgtgcctcctcatccagcctgccagaaaagaagatgcaggatggtacacactgtcagcaaagaacgaagcagggattgtatcctgt
actgcaagacttgatatttatgctcagtggtaccagcagattccccagccaatcaaaaagacccgatcaacaggttctcggtattctatgttttcagctt
tggatgccatcagctctcatcctcctgtagtaacaccgctgaattactcattgtctacacgggaagtggtagaaagtgaagagctgtgaatggaaccgac
gtcaaaacccaaaataaaactgtgaagaaaatacagcttgaacgtttacaaatcaaatttttaatggggataaagatccctgtttggcttcagtgttttg
ccaatataaaataatctaactgccaggtagtactgtttcagtgccactagaggggcccagccctttctggcagtgaacaggttaacataggtttgcagta
tatgtagcccagacgcgccagcagtactcagtgatctgtatgcccgtggcactgacactgatgcagtgtgatctgtttgatgttgtataagtaccctatt
taaatatttatgtcccacaaggaa
3' EST: DT534191 [+]atgcaacatgttgtaactttattagggaatacatcccattttcacttcctataaacaaaccgccatttgttaaatagcaagagatggcacttttagttta
aaccctctactgtataagcaaccttgttttaacaaagaatctggtaaaatacaaataatgcacatttatatattaatataacctctataggtctttactt
tcttttctttgtggacataaatatttaaatagggtacttatacaacatcaaacagatcacactgcatcagtgtcagtgccacgggcatacagatcactga
gtactgctggcgcgtctgggctacatatactgcaaacctatgttaacctgttcactgccagaaagggctgggcccctctagtggcactgaaacagtacta
cctggcagttagattattttatattggcaaaacactgaagccaaacagggatctttatccccattaaaaatttgatttgtaaacgttcaagctgtatttt
cttcacagttttattttgggttttgacgtcggttccattcacagctcttcactttctaccacttcccgtgtagacaatgagtaattcagcggtgttacta
caggaggatgagagctgatggcatccaaagctgaaaacatagaataccgagaacctgttgatcgggtctttttgattggctggggaatctgctggtacca
ctgagcataaatatcaagtcttgcagtacaggatacaatccctgcttcgttctttgctgaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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