XB-CLONE-3054409
Clone Name: IMAGE:7849097
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534257 [+]
caaacatctaacaatccacaatgggaaaggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatct
tatctacaaatgtggtggtatcgacaagagaaccatcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttg
gacaaactgaaggccgagcgtgaacgtggtatcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccag
gacacagagacttcatcaagaacatgatcactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtat
ctcaaagaacggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaaccc
ccatacagccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctattt
ctggatggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactac
cctgctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggt
actgtaccagttggtcgtgtggagactggtgtccttagccaggcatggtggttacttttgcccctgttaatg
3' EST: DT534256 [+]tttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaa
tgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaag
agttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactt
tgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtc
agagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttacca
gaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctg
ggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcac
agaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacatta
acaggggcaaaagtaaccaccatgcctggcttaaggacaccagtctccacacgaccaactggtacagtaccaata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: