XB-CLONE-3054360
Clone Name: IMAGE:7849149
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Gene:
myoz1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534289 [+]
caagcctgctggagtccgatatttgaattctttgtagctgcaccgaagtgaaagtgtacaagagaagaacgttaaagaggccagaactaagtgcagaacc
attgcatctttgttgacagatgcaccaaatcctcattccaagggtgtgcttatgtttaagaagagacggcagcgagcaaaaaagtataccctagtgagtt
acggaagtgtggatgaagaccgttacatcgaggacgaagacggtgtatttcctacaagtgaatcagaatttgatgaggaagggttctcagatgcccgtag
tctaacaaatcactcagactgggacagtacctatttagatattgagaagccaaaaatggatgagcagcaggtcgaaaagggattgactgaggcatcaggg
aaaggagctgagttatttgagctacagagacagcgatcagaacagtcccctgttgattctgttcctttaccaatccctgttcctttacaacaacgtaaag
agaatcaaacagctccagtagctccagtccggaaaaagatgttaaatggaaatgcaaactcttcagctattgtaaataaaacccaagaaatgggtgtttc
tgaaagccccaaagtagcaagtactaatatatcaataactcctcctcagtcacaacccactcaagtagatggtgttcaagccagttctgaaatctttaat
agatcagcacgaccatttaccccaggtcaaactggccaacgtcttgtggcatcatcagttgtattcaagcccagttccccnaagaaaacaagtgaaattc
caccatctcaaatcataactccttcacccatcccatcattcatcccagaaacacctttaaatgctg
3' EST: DT534288 [+]tttttttcatttaattgttgcattttcttttgtttttaaggcagctggcacattatagtctttacctactaagcatgtgtcctgtagtaactacacaagg
agcctgatgctcattgtttaagcaatacacaaaataaataagcaaactatttttccattaaccttttcaggctacattcctggttagaactttactttgg
ctggcctccaaacattagcctgcatccctgttttggctgcacagaactttggctttgcaacttggaaagttgaacttgatgatctgggagaagtaatgga
actagttgggctaagtataccataactctggtagctagaagggatgaaattctgtggagtcttctgttcaggaacttgacattgattaggcactgtaacc
actgatggcataggttggcaggtaagatctgttctccatactggctctgcaagagtagttgcagatcgaggagttatcacagttggtgtcatactgattg
atgctgtcattggaactggagttgaaaaacgttttatctcatgagccctagcagttttcacaggaacttgtctagctggtgatgccaatacatttgattt
ttgtgtgtctctttttgttggagtctggtttgggctgttggaagaatcatcaaaaacaaacattgcagaatttagctgatatggctgatgccgcataaaa
tctattgcctgcattctgggtttgccactgggacctttcttaactttgcttgcaactttgctagcttgagtttttgatgccgctaatggataaaatggag
agcaaaggggattataggcaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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