XB-CLONE-3054348
Clone Name: IMAGE:7848959
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Gene:
sod2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534297 [+]
cttagatgtgctccagccttgacatactttacgtccagggaaaagcatactctgcctgacttaccatatgattatggtgctctgcagccccacatcagtg
ctgaaatcatgcaactacaccacagtaaacaccatgctacttatgtgaacaaccttaatattacagaagaaaaatatgcagaggccctggctaaagggga
tgttacaactcaagtctctcttcaagctgcacttaaatttaatggtggtggccatatcaatcacacaatcttctggacaaacctttcccctaatggtgga
ggggaaccacaaggagaacttctagatgccataaagagagactttggctcctttgagaaatttaaggagaaattaagtactgtgtcagttggagtccagg
gatcagggtggggatggttgggttataacaaggaatctaatagacttcaactggctgcctgtgccaatcaagaccctctacaaggaacaacaggtcttat
acctctgcttggaattgatgtgtgggagcatgcttattacttgcaatataaaaacgttagaccagactatatgaaagcgatctggaatgttatcaactgg
gagaatgtagccgaaagataccgggcttccaaaaaataaagtgctagatctgttctctgttctgtccgttacagccagtgtaacacatctatacagcatc
tatagttaaagattatctgtctgttttcaaagagaattgttgcttctcacacatccctcagactgaatgcacttactagacttacaggccacgtccaatc
tgtgagcacttagaagcctgtagaatcaatc
3' EST: DT534296 [+]ggaagataaaggttgattntatatatagcggaaatataaaggctgtgatttagaaaaagagaaatcagactaaggaaataaacatattaatggtgagttt
tgctaacacttttattgtgactgtaattttagtagttttggttcaaataggtgttttataataggcatatgaggccttggagaaaagcagttgagaacca
tagctctagctttctgccagttaaaaatctaatgtcacatgatgacattgattaagtgaattttcatgctgtacttgtcaaaaatataatttatattaca
aaaaaaaccaatttactaaggaatcggcacattgttcaacaatttatagttattaaagaaatctacacatatagaaaagtatgcaagaaaaaaattaaca
ttgctgctgcctttattattgaattgcaaaatgttattttcacaacagaccaccagtaaagctcaagctatgaaaaggattgattctacaggcttctaag
tgctcacagattggacgtggcctgtaagtctagtaagtgcattcagtctgagggatgtgtgagaagcaacaattctctttgaaaacagacagataatctt
taactatagatgctgtatagatgtgttacactggctgtaacggacagaacagagaacagatctagcactttattttttggaagcccggtatctttcggct
acattctcccagttgataacattccagatcgctttcatatagtctggtctaacgtttttatattgcaagtaataagcatgctcccacacatcaattccaa
gcagaggtataagacctgttgttccttgtagagggtcttgattggcacaggcagccagttgaagtctattagattcttgttataa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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