XB-CLONE-3054321
Clone Name: IMAGE:7849009
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Gene:
slc12a6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534315 [+]
caggaccccctcgtaaccctcagggtgatgagaattatataggagtttctagaggtgcttactgagggcttggaacacgtgctgttagtgagaggaggtg
gaacagaggtgatcaccatttactcataggtgtgcacagtgcatttcttaggttggcattgtggaaaccaggaaccaacctgagagagagtggaaagatg
gaacttttgttaatgccaaattattccactcccttgaatggaacaaggtgaaaattctcagaagtgttggcattaaagatcttttcccgtaaattgtagt
tttcataagggagtcaccaaaataccgtttaatgccaaaggggagtgccatgaatgcatttgctgtccaaagaatctatcccaaacacctctggctggaa
aatacggaaaaagacttctagctctcacccagtgacaatattcatctctatgcagcagtgctcctgccttttcaatttgtaatgcttaatgtactactca
gctgaagatatccagattatttaagacatatctttgtacaaacagattagcaccttcttcattagtaatatgcaaagttactgtacgcatatagctcagt
tcatttttcgtgatccagatgttccaaatgggatcaaagagctagtacatgctaagcaagttaagtgattctaaatactagcttaagggaattcacgcgt
ttgtgcatattgtgactggacaactcagctttatcagatctctttataacccagtgaacaccaaaaatgatctaccacatcggccacaaaaaccttgggt
gttctaatgacattcctgtaaaagcttagc
3' EST: DT534314 [+]tatagggcgagaggttttgacatgaaagattttattgccagccatagaacaggctagacagaaggcagctgctctatagtgcagctattgctagtttcaa
tacttttggtacctactctatccagcaatacaacaaagaaatcgagacagcagtcatagagtgaacaaacacatagcatgatgcactgcacaataaatta
aaacctgctcatttagcatgttgcataaaaaaacatgtagtgcaaatttaaataaaactagtgggttttcccatcccttctgaatatgaaactgcaatga
ataaagttaagttactgctactcatgctaagtccatgagtccacatctttcaaagtacaattctctgggccaaacaaagattttggtgtagtagtctcga
ccagtaatgctgtctgaggaatttccttatacacagttctaatcatgacagcatacatttaaaaaaaaaaaaaaaaggctaagcttttacaggaatgtca
ttagaacacccaaggtttttgtggccgatgtggtagatcatttttggtgttcactgggttataaagagatctgataaagctgagttgtccagtcacaata
tgcacaaacgcgtgaattcccttaagctagtatttagaatcacttaacttgcttagcatgtactagctctttgatcccatttggaacatctggatcacga
aaaatgaactgagctatatgcgtacagtaactttgcatattactaatgaagaaggtgctaatctgtttgtacaaagatatgtcttaaataatctggatat
cttcagctgagtagtacattaagcattacaaattgaaaaggcaggagcactgctgcatagagatgaatattgtcactgggtgagagctagaagtcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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