XB-CLONE-3054274
Clone Name: IMAGE:7849013
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Gene:
myh13
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534346 [+]
aagccaagggtgagctgcaacgtagtctgtccaaggccaacagtgaagtgtctcagtggagaaccaaatatgagactgacgcaattcagcgtactgagga
actggaggaagccaagaagaagctggctcagcggctccaggaagctgaggagcaaatcgaggccgtcaactctaagtgcgcttccctggagaagaccaag
cagagacttcagggagaagtggaagatctgatggtggacgtagaacgttccaactcagcttgcgcagctcttgataagaagcagaagaacttcgacaagg
tgctggcagactggaagcagaaatttgaagagtcccaggctgaacttgaggcctctcagaaagagtctaggtccctgagcactgaggtgtttaagatgaa
gaactcctatgaagaagcattagaccaactggagaccctcaagagagaaaataagaaccttcaacaggagatctctgatttgactgaacaagttgctgag
gctggaaagagtattcatgaaatagaaaaggccaaaaagcagattgaatctgagaaatcagatttacaggcagcactagaagaagccgagggttctttgg
agcatgaagaagccaagatcctcaggatccagcttgagttgaatcaagtcaagggtgagattgacagaaaaattgcagagaaagatgaagaaattgagca
gctaaagaggaacagccagagagctctggaatccatgcagagtaccctggatgctgagatcagaagcaggaacgatgccctcagactgaagaagaagatg
gagggagatctgaatgagatggagattcaactaagccatgcaaaccgacaagcagcagagtcacagaaacagctcaggaacgtgcaggggctgcttaagg
atactcaactgcaccttgatgatgcacaacgagg
3' EST: DT534345 [+]gaaccccaaaaggatttatttgcgtacagaacactacacaactacacacatacacagggtctcactctacagaagtccatattgtatgaactggtgcaga
tagtctgtgcggctttcactcttccgctttctttccaatatcccggctcttggctctgagcttgttgacttgggattcggcaatgtcggcacgttcctcg
gcctcttccaattcatgctgagccttcctgaatctggacaggtggacattggcttgttcttcaacatcctctgattgtctcttgtatgctttgactttca
gctgcaacttgtccactaagtcctgaagcctgaaaacattcttcttgtcctcctcagactggtaactgagctccttgactcttctctcaaacttgcgcac
gcctttgatagcatcggatcctctcttctgctcagcgtcgagctcattttcaagttcacgaaccctggcctccagcttctggagctgcttcttgcctcct
ttcattgccagctgctcagcctcatccagacgatgttgcagatccttcactgtctgctccagattcttcttcatacgttcaaggtgagcactggtgtctt
gttccttcttgagttcctcagccatcatggccgcatcggtgattgccttcttggccttttcatctgcattccttgcttcctgaactgcttcttccacctc
actttgcagctgagagacatcagcctcaatcttcttcttggtgttgatcagactggtgttctgtgagtggagaagctgcacgcgctctgtgacatcaatg
agttcctgttctgccactttgcgcccaccctcagtctgttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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