XB-CLONE-3054181
Clone Name: IMAGE:7848973
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Gene:
usp13
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534408
[+]
ccgaggcttctcagtgctgtcagctatgatttatttaataaaggggaaagctctgcatgtttccagctaaagagcccttcatgcttttaaggtcgtgcag
accttgcagaaaaacgtaagatgacgatttcagagataccaccctaatgttctgaggtcttgttttcagcggcagtaacttgctgattcattcagcacta
ggtagtattctctggcgagcaggcagcgagcgggtagcatgcccatcttgtgtcagaatgatggaaacaggaacctgtaatctctacatctccatatgac
ctacagcttccagggtaatgaaacttactggtaactgagagattgtgtaattcagctggttagtggaaaatttaatgctagagtatgatttgtttagatt
tgtttatttctttcccttgttacagtggtgaataaaatagttttccatggctaattaaagaattgtagacagtgcagtcaaattattatctatcagtaaa
ttttactgagcagtaacggcctttattcccagcaaaacattaccaacacatacgggtataacagttaaacaatatttctgatgtcagaatagattataga
atagatttaactgctgggaatcattctaagttcctacatttaatctgtaataaataagccccaatgtttcagataatccggagtatggaggttctgtctg
gttgagcaggtgtgtgtatttttttatgtgaacatttgtgtgtgtgtgtgcctatactaacacactattgtcactatgggtttttttaacagaatattag
cactatattttaacaatatgtgctagatattctcatgagtgacatgactgtgtctactgtatgtgggtaaacatttttaaagattttaaaagaattctct
tgatttttc
3' EST: DT534407
[+]ttttttttttttttggatgtgcaaaatgttttaattaaaataatttgcaacaatattcatcccttctgagcatatttttcagcagtgacttaggcatttt
tttcccagagagatagaataaatgtgaatacagaagtataacaaaatatcataacttatttgccgaggatttacaatgtctttaaacccataacattttc
gagactgttttgcaagggatataaaacctgtggctctcctgctcttgatgaactacaacaggttgaagatccctgggttaggcagcttctgcatagtacg
gcaatgctgtaatacatagtgtggccgagtaccgtagttaaagctataatagacagtagtccagtgatcattaacctagggctctcaagttgttttcacc
caggttggattccagtgctcttattacccaggggttgagaaaacatttgtgtcagacatgcaaactatatgtaatccagatgccagatcaagagatgggt
ccattggaaaaagtatttactttgatctccctatatgctaatttttaaggttaaaaaaatagcctacattatagagtgtcagtcaaagaacaaaatgtcc
cattaaacagttaagtaatgagtgcgctactgaatacagttctagactactgtcagatgtgtaacagagaaagcccttaaaaatggtgtggttgccctag
ctgtaaatgcaaataaatatttaatttacaaattaacccttccatacttaaatattcctttgtatctcattacattaatgaaagagatgtatggggttta
aggaccatttggtttgatttttctttgttattttgtgaggatcaacaaccctggatccccctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: