XB-CLONE-3053892
Clone Name: IMAGE:7849000
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534598 [+]
actctttgctgtaactccgacgctgcccgagctctgagggcaaaggagaaccaagccatggccttaccgacccagcaggctgatgagttgcgcctctacc
agcaaactctgctccaagatggactcaaggacatgctggaccacgacaactttatagactgtgttctaaagatccagggcaaggagtttccctgccaccg
cctggtgctggccgcctgtagcccttacttccgagccatgttcctttccaacctggaggagggcaagaagaaggaaattgacctagaagatgtcgatcca
gacgtgatggggaagatcctccattatatctacacttctgagatcgagatcacagagaagaacgtccaggacatattctcggtggccaacatgttccaga
tcccctcgatattcacagtgtgtgtgtctttcctccagaagaagctctgccttagcaactgtctggccatattccgcctgggcttgctgttggattgtcc
ccgtctggccgtctccgccagggactttgtatgcgaccgattccacctgattgccagagacgaagagttctacgacctttcccccgatgagctgattgcc
gttatctccagcgactccctcaacattgagaaggaagaagaagtctttgaggtggtcctgaaatgggcgttgaaagagaaagaaaaacgcgccaaggcgt
tgcccatcatctttgagagcattcgcttccgccttataccccaagactatataaagaacaaggtggagaagcacgagttggtcaagtccgacaaggaact
cttgaagaaactccagatggtcaaagacg
3' EST: DT534597 [+]gcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaatcc
ttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgaccttt
attgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaatttag
gtacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttgaaa
atcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaatg
gttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaagat
tcatccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatgata
taatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtaaaaatgaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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