XB-CLONE-3053835
Clone Name: IMAGE:7849196
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534635 [+]
aaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatctacaaatgtggtggtatcgacaagag
aaccatcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggt
atcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatca
ctggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgc
cctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatc
gttaaggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagc
ccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctggaagctcttgactgcattttgcc
accaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtggagactggt
gtccttaagccaggcatggtggttacttttgcccctgttaatgtaacacctgaagttaaatctgttgaaatgcaccatgaagcccttagc
3' EST: DT534634 [+]tttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactgggatttaaaacactaataacttaaaaactgccacaa
acaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgata
gaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgt
gactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagagggggg
tagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttct
taccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctg
gcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtcc
ttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgtta
cattaacagggggcaaaagtaaccaccatgccctggcttaaggacaccagtctccacacgaacaactggtacagtaccaataccgccaattttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: