XB-CLONE-3053806
Clone Name: IMAGE:7848912
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Gene:
zfp36
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534654 [+]
cttgccaattcttcggcagaactgagttctgtctctgtagtcaatgtgtttttgcatatgattgaaggaaaactatagccaaaacactagctgcttctat
atgaataagtattttgcaacatacatttgtggaagtggctcttcacctcctactacaattttattgtaaaacacagccagatattagtcttagagatcat
aaagttgatagtgcaaaacaaaactgttcctgctcaacatcttaaaaagttcttaaataatatttgctatattcttcaaagacacctatatatatatata
tttatttatatatatatatatatatatatatattcatactttttcacataggagcatcatgtgcctaagctatagtttttccttaactttttttccataa
tatgcatgaaaccttttcagaggatacatccctgtgtctcagagctgaaagatatttattatagttattatgaatattgtttaagggccttagtctctca
aatgtgcaagtgccctgaattatgtagcagaacaaggtacaatgtgcaggcctccagttgcaccctgtgttttggctggttccaaacatacagaagtgtc
acttggtctggacctgtagctgtgccctggaaactccacccaaccccagaattcctatcccattgcagagggaagtactataatgtgtagctagcctaat
ttacctgagcttctgcccacagctcacagcttcatgtgcctctctacatataatatccaagcacttgctgtagataaactctcatctaaacatagcatgg
acacacactgatgtgaataatagtacagatgcactctgctctttgtgtaatagagacataaccttctctgtgcaaactaaagatacgtg
3' EST: DT534653 [+]gtatgcaaatctctttatttcagattataaactcattgcaaatgctgacaaatagatattatttacaaaataataaaaaatgataaatatatttttcatt
tttaaaaataaatatgatataaatagattaatacacgttagaagaagcacactttaaggttaaagatcaaggtatgaattagtatcaggttttgctgata
aagtttctctgctccctacatttcctgtttgtgcctgattttaactccttgattgccagaaaggacctgtcagtgtaactgtgaggtaggacacacattg
ttaggaatgaccctttgctgctaggaagtactgcaatgcgtgcctaaccctgcaccatggatcatcaaagacagtagcatagtccaatgcaatggagttt
aaaaactagcaagcactgaatatgatctgtgtgtgtctacagtctgtctgatgggctgggtgctgtattataagcaaagcagcatgtgctgaagtttctt
atacacacaacactagaaatatcttttacacagaccaatatgtgcattatacaaagacagggtacatcgatatttatgtatatagatatatatctatatt
tatgtagagcagtatctatgttaaaggagccacgtatctttagtttgcacagagaaggttatgtctctattacacaaagagcagagtgcatctgtactat
tattcacatcagtgtgtgtccatgctatgtttagatgagagtttatctacagcaagtgcttggatattatatgtagagaggcacatgaagctgtgagctg
tgggcagaagctcaggtaaattaggctagctacacattatagtacttccctctgcaatggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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