XB-CLONE-3053740
Clone Name: IMAGE:7848870
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Gene:
ppp1r1a
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534698 [+]
tcactgtcccactgctggagccacatctggaccctgaagctgcagaacagatacggagaagaagaccaacaccagccacacttgttctgagcagtgacca
gtcatctccagagatagatgaggaacgtgtccccaaccctttacaaaagtcttctttatccatgtccccacatcagaggaaaaaaatgtcccgtacaaca
cccaccatgaaagaactacaactattagcagaacatcatctgtgcaagcagggatcagaggaggagaagatcccccatcttcagaacaacctggatgaca
ggcctgacttgggatgctgttgccatggtaacacagcctccactcaagagtcgcatataccatgtagctgttttacccaggatggacagcaagagaataa
ttccctgggaaggagacatgctagtcaggaggattcattagacagtcttgtgtcagatggaaatacgcaaacatgtgagccaaaaaaacagagcagccgc
atagctttcatagaagacaaataggtctgaaaaagcaaaaggatttcttattgagtataaagggagtgaggagccaaatcccagcacagtaccgtgggct
tacagcacataaaggaccgacagctccctccttttaaaaatgatttttccttacctctctgctactgagaatctggagtctgcactgagttctgagcaat
aaactgcaggcttctctggaagcaaagaagttcaagaaaacattgctttgtgtttgatatcttgcctctgtactgtatgtcttctaccttttcaggggaa
gtgtgtatctgtatgcagaaagccaaactggccgctgctatgccaatcattgtta
3' EST: DT534697 [+]aaagggcttcctccattttattaagaatttgacattataacgaatcgcatctggcaaatacatgttcccaatgcaggtgctgcagttgtggagcagtgtg
gggatgtggcacgtttagtgatctttatttaaggtattgcaggtgagctttctgtaagcattccttctatagatgtagctgctaggtacagtgtacatta
atctacagtctttcttgggcctaggtattttttgcacacacaaagttattttttcatttaggtaaaacccaaaaagcacaaatgattttattctacccac
atgttatatcctcccgactcatcctgagggctaataagggctaacaatgattggcatagcagcggccagtttggctttctgcatacagatacacacttcc
cttgaaaaggtagaagacatacagtacagaggcaagatatcaaacacaaagcaatgttttcttgaacttctttgcttccagagaagcctgcagtttattg
ctcagaactcagtgcagactccagattctcagtagcagagaggtaaggaaaaatcatttttaaaaggagggagctgtcggtcctttatgtgctgtaagcc
cacggtactgtgctgggatttggctcctcactccctttatactcaataagaaatccttttgctttttcagacctatttgtcttctatgaaagctatgcgg
ctgctctgtttttttggctcacatgtttgcgtatttccatctgacacaagactgtctaatgaatcctcctgactagcatgtctccttcccagggaattat
tctcttgctgtccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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