XB-CLONE-3053661
Clone Name: IMAGE:7849020
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Gene:
alas1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534750 [+]
ctgccattcacttctattcgtgctattgatttgaaatccccgtcgataatggataccattgtgcgccgctgtccctttctctcccgtgtatcccagacct
tcctgcagaaagcgggcaaatctctggtgacctatgctcagaactgccccgtgatgatggggaccaagcccttgtcatgtgcttcgttgagtacgtcggc
tgttcatcagcaagagaccaaggagacatcagattccaagacaccacttaacaggacccaggttcagggaggcctaactggatcccaagtgccagcagat
caccctggcgcagtaagtgggcagagtgcatcaaaatgtccttttctggcggcccagatggtgcaagaaaacagcagcgtcattcgcaaggccagccctg
agactcaggaggatgtacagaagatgcaaacaatgagaaaagatttgcgtgggccaatcaccaaaggtgccggagaagcaaaaatgaccctgcagaaaaa
tcgtagcgttctagagaaatttcaagagagcgtgcttcatcagaggcctgcagtagtttcccatcttcttcaagataacatgcctaaagctgtaaagacc
ttcaagtatgacaaatttttcgaaaagaaaattgacgagaagaaaaacgaccatacttaccgcgtcttcaaaactgtcaaccggcgtgcggaagtgttcc
ctatggctgacgattactccgacactttaataagcaagaaaactgtctctgtgtggtgcagcaatgattacctgggaatgagtcgccaccctaaggtggt
aggagcagtgatggaaactcttaaacacatggagcggggccggcggcaca
3' EST: DT534749 [+]ttttttttttcatggtttgcaagacaaatatttttaattcattaagaaaagataattgtcctgtaatacaatattcataatataaaacacaggaattatt
agacaagatacccagtgtgacataaaagtgtatacttttttgactacgatacattatttcatctctatttaaatgtagaatttgttttgttatggggaca
ggggttgtgtcaagctcttacagatatcatcttactcaagcctgagaaataggatttctcgcgttcgctcatcacctcgaagtggagaggctgcctgcag
aagttacattcggctgtaggatgaggtttcagctccaggtccacactcttccaggtggtcagcagcttgtccaagaaataactcatcatctgagggttgt
ggtgaggtgtgggtgcaatcctcaggagttcctctccacgcgcaacagtggggtagttaatggcttgtacataaatgttataatctctcatcaatttgtc
gcaaatctcagtatttttagccgcatcagaaaccctgatagggataatatgactcggacaatggaccactgggaggcctgaatccatcagcatctgtctc
atgagcttaacattgcgctggtgctggcggcggagagcttgaccttcttcactcttcaggactctcacagatgctattgctccagagagaagcatgggcg
gcaaagaggttgtgaatataaaaccagctgcgtaggatcggacggtgtctatcagggcacttgtactagcgatgtatccaccaacacagccaaatgcttt
gccgagtgttcctgaaatgatgtccattttggccattactccatcgcggtctcccatgcctcctccccgtgcaccggtacaatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: