XB-CLONE-3053652
Clone Name: IMAGE:7849164
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Gene:
slc25a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT534756 [+]
aaatgtattcctccgttgtgcagttagcgcggaccaaccctttcaatgctccccactttcagttggggcaagattctgtaatatcaaggaaaggcaatat
cccccaaacaaagccgacccgtcagcttgctgctgctgctgttcaagaagaggagtatagctgtgcttacggatctggcaagttctttgcactttgtggc
tttggtggaatcataagttgcggtacaacccatactgcagtggtcccattagatctggttaaatgcaggattcaggttgaccctcaaaaatacaagagca
tcttcaatggcttttccattacccttaaagaagatggtgttcgtggccttgcaaagggatgggctccaacgttcattggatattcaatgcagggcctgtg
caagtttggtttctatgaagtgttcaagatactgtatgctaatatgctgggcgaggaaaattcctacatgtggcgtacttctctttacctggctgcgtct
gccagtgctgagttctttgctgatattgctctggctccaatggaagctgcaaaagtacgcattcagacacaaccaggctatgctaatactttaagacaag
ctgctcccaagatgtatgctgaggaaggcatctgggcgttttacaaaggtgttgctcctctttggatgaggcagattccatacacaatgatgaaatttgc
ttgctttgaacgaactgttgaagctctctataagtatgttgtgcccaaacccagaagcgaatgttcaaaatctgagcagttggttgtcactttcgtggct
ggatatattgctggtgtcttctgtgccattgtctcacatcctgcggattctgtggtctctgtactgaac
3' EST: DT534755 [+]gtgtttgggatggatatttagttctgtacattaaatctttaaccatcagtttgttaagtttaaattacataaatgcaataactctcatctgtttatttca
acagataaacacagcggtaaaaccaagttacaatgtgtaggctatgcggcaaggtgagagcacaaccaataattacatctgaaacaaccataggtcctat
tttttagttgaatttataaaaaacgccttttgaaaatgttggagtttgcatgatttgagtccatgtttactgagtgagaccaagcttcttcttcaaggat
tctggcatctcagggggaggaggacgaggaagtctgaagtaaaccttcacagagtcatagataaaccactgcaaagcagtcaaggtaccaatcatgatga
tacgggcagtcaaacccttccagacacctttaggcccaagtctcttcagaacttccaaagcagtgcttcccttttccttgttcagtacagagaccacaga
atccgcaggatgtgagacaatggcacagaagacaccagcaatatatccagccacgaaagtgacaaccaactgctcagattttgaacattcgcttctgggt
ttgggcacaacatacttatagagagcttcaacagttcgttcaaagcaagcaaatttcatcattgtgtatggaatctgcctcatccaaagaggagcaacac
ctttgtaaaacgcccagatgccttcctcagcatacatcttgggagcagcttgtcttaaagtattagcatagcctggttgtgtctgaatgcgtacttttgc
agcttccattggagccagagcaatatcagcaaagaactcagcactggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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