XB-CLONE-3053278
Clone Name: IMAGE:7849485
|
Gene:
zw10
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT535003 [+]
caaagttgaaattcttcagccagttgttgctaaattacatattgaagcccctggttacttatccatccctccatgcccagatggaaacacaacaacaggg
tgttattttacgctttgaaagtgttgagacacagttggagcacccacctccttctgaggttttcatcaaactgaggctggtctttgaattacttcataag
cacctattagatgtgccagtggaagattccccacaagctggtgagacaggcaaggtggtattagcagaagttcttggtgaaatgatttgggaagaaatct
ctgaagccatcataaaagattgcttagtctactccatcccaaataacagcagcaaacttgttcagtatgatgaggttataaatgcaacagaagagtttga
aaatgctctaaagggtatgaattatcttaagggtgatgcaactgatcttctgaaatatgcacgaaatgtcaatgcccactttgctagcaagaagtgccaa
gatgttattgtcatagctagaaatttgatgacctctgaaatacataacacagtaaagattacacctgattccaaaattactgtgccaacactctcaaaat
atgaaggttcggagaagataataaagaaagagaaccatctttcgcaagaagcccctggcctggagaatgatgttaaactgagccaacatacatttgctct
ccccacttgcaggatcagtgagtctgtgcagaagttaatggagcttgcttaccagacactctcggaagctactactagtagtaaaccatgctccatccag
ctattctacacagtcagaaacattttccatctgttctatgatgtagtgccaacgtatcacaaagaaatttgcagaagcttccacagctttctgcaatcca
ccataacaattgtatgtacattg
3' EST: DT535002 [+]cttaagaacttgtttattggcgttcacagaagcatactttatgtacaaaaatatacaggcattattgtgaaattgctatagaagttatttgatctttgcc
aatgcagccgctcttctttctgtattctgaaacagtgctcgaattaggttcttcacttcactggtggaaaactccacagcaaggggtccttttccatctg
cccaccgatccactatctcctgcaaactggcctgcaatataatcatttgttctttaaatttcatccacttttgaacatagagaggtacttcttcctgata
ttttttgttctttgcttcttccgggataggactgaataaaagtgggccttcctctatcattgtccggcataatgtgtacagacgttctccatcttcagta
gagatgtcctctaaagcagtaattttgctgattatttccacaatagcagtgtttagtaaggtgcccatagctttgcagtatatagctataggaagaacat
cctgccataccttacccaagcgactcagctgatggatcacctgccttattgctttgtgagcagctgtataattatcttcatcttccatgttgctgaaatt
tcttgcatttgaaagtctttctagaagatcagctttttgggaacgcatttgggctaggaaactttcagtcccaagccttctaaatcctggaaccatgtct
acaaaagtagccgctccatcactgagtggggaagggaggtggtaacgaaactggtgtccaagtgtcagcagatggtgtgcaatgtacatacaattgttat
ggtggattgcagaaagctgtggaagcttctgcaaattttcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: