XB-CLONE-3053264
Clone Name: IMAGE:7849343
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Gene:
taf8
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535012 [+]
attttcaaaatcttttattatgaaacacctcatgagggacagtatctgtgtcctcaaatcagggtattaaatgaaaagatatatattatatcagggtatg
aaatgaaagatatatatccctattcaaggatccttataaggttcaaacattgcatgtcaagactcaaattaaagttgcccatatggactcttaacttttc
ctgtaatcaacaatacagagcagccaactttaataaaatttcaatatcaacatccttttcaagctctggtaaatggagtcactaatgtccagttaaatat
tctgtagtttttcattcagaatctcagggttggctactctatttcttacgcctcagcttaggttttttcacaggtcgtagatagggattatctataagtg
tttcctctccacccttcattgaattctgctgtaaaacagatgaattttctttctccatgcccacctcgtccccttgcagatgcatagacaggttctctgc
gtcagtctgatcatcttgctctgaggaatcagtctcctctacttgctgcaattctagttctgatgggaggagggcattcagataaggtattgagagtgga
cgtgctgcaatcaacggaaaggtgcttgtgtcatctttgaacaaactctgtgtctcgcctgttttagccataaatcgggtgagggccctttccacatctc
tacgctgtgatgctgccttctcacgcaagacttggtaatcacatactggttcacgataagtcggtgtcttaatgtatgtatggggatctggaaactcggg
aaaatggcttggaatatgagcagggtgttgcttgttctgtcctgcactcagagcttta
3' EST: DT535011 [+]tcggattggatgactccttttgttattttttttctttttaatggcgatatttacatggtagcgctgaaaagaaaagggaagatggctgccctgtggaact
ttatttgatacgctcgtaatggtacaaagggaaaagtttttgttccagctcttttagaaatcaagctgcagtcaaaaagactgtctcctgtactgaggtg
ctagaagttgtatataatacagaaacgctttttatgtttcctacaattaggtcttgtgcacgtgggacttccacccctgccgataattacatgttggcga
gacgaaggacactgcaggtggtagttagttccctgctcacagaggctggatttgaaagtgcagagaaagcagctgtagaaagtttaactgaaatgctaca
gagctatctgtcagagattggacgaagtgccaagtcatactgtgaacacactgcacggacacagcccacgctgccagacattgtagttactctgatagaa
atggggtttaatgtagaaagtttgcctgcctatgccaagcggtcccagaggatggtgataacagcaccacctgtgacaaacaatcctgtggttcctaaag
ctctgagtgcaggacagaacaagcaacaccctgctcatattccaagccattttcccgagtttccagatccccatacatacattaagacaccgacttatcg
tgaaccagtatgtgattaccaagtcttgcgtgagaaggcagcatcacagcgtagagatgtggaaagggccctcacccgatttatggctaanacaggcgag
acacagagtttgttcaaagatgacacaagcacctttccgttgattgcagcacgtccactctcaataccttatctgaatgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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