XB-CLONE-3053215
Clone Name: IMAGE:7849395
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Gene:
myoz1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535044 [+]
catcaaagaagctcccatctctcctcctttagctgatctccagttcctgcgaaaaagaattaagaatttgttgtaatgccactgtcaggaacaccagctc
caaacaagaggaaaaagtcaaccaaaatcatcatggagataaatccatcaagagaaggagagatagaaatctccaaactcaatttagggaaaaagatcag
tgttcccagagatatcatgctggaggaactatccttgctcagtaacaaaggatcgaagatgtttaagcttcgtcaaaagagggtagaaaaatttatctat
gaaaacaaccctgacgtattttccgacaattccttggaccacttccagaaatttataccaactgtcggtggccaattaggggctaaaggacagtcaggtg
ttggagtaggtggctatggattgctatatggcagtggtcagatgctatccagtatttcagggggacggcatggatctggtggtcagcaacctcctgtacc
tccaccaaaacctggcagtaagagcgctcctggaggccagtctggaactggaggaagtggtggaaaggattctggtgttgttgatggtagtcaaggaaaa
gagggacagtcaggtcaacaggatggaggaagtgggaagcagatcactgtctttaagacatacctctcaccctgggacaaagctatgggcattgacccta
agcaaccaacacattttactgtcaatttactagagtatggcaaaaaggcagagctttgcaaatataagtcatttaacaggagtgctatgccttttggtgg
atatgagaaagcatcgaagcttatgactttccagatgccagattttgatgccccccctcagagcctgagcctgtgggtgtc
3' EST: DT535043 [+]gatgttgtaaatgtttattcagtttaacattgcacaaatgaaaaatatcaaccatacagcatgactgaggagccaacagtaaacttatttggagactgtc
cttttatcgctgccacagtgcacaaaacattaagcttttttccttccactatcctaccttgtctattccatttacctctgcccagttccaactctgacta
acttccagctgaaaaaataccaccataacaaaaaacatttcataaacatacataaaggagctttgctgcattttgaacaaggcacattagagtgtggcat
agttgggtctaatgtgatctgccccattttttaaaaaaggcatataaagatttggcttaggtggtttagatatgacctgtgccattggctgctatggcat
cacagcatactgcaatgcattgcttgtaccacagatatttcagggatcaaccgccaatgtgtttgcaatatgttatgatgcagaggccggtggaactttt
tacatatgtcagttttcatacaccaacagactgctaacatggttccttggatgtatgatgagtttagagttcttctgtttctccatccactggcacagca
atatcaatgctgtagtttgtgttatcaccagatccaagccatccaattggggtcctgttgaaagaaggtctgttggtgatatcttgattgatgacaacca
caggctcaggctctggagggggggcatcaaaatctggcatctggaaagtcataagcttcgatgctttctcatatccaccaaaaggcatagcactcctgtt
aaatgacttatatttgcaaagctctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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