XB-CLONE-3053209
Clone Name: IMAGE:7849491
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Gene:
rasgef1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535048 [+]
tacagacctgggcagcaggttctcgctaagaaatcgctctggaaattctgcttgtatgtgaaggaaccggcagggatttcctgaggtctccggttggctt
tccttttcttacgaattataaccctcttttagtctgatcaatgctaatcggatgtgtcggtttctaatcgcagcagtttggagtcttacgggcgtgtaag
cgatttaataactgctacagtatcacccggctgtgtgcggtgacccaggaatttctgttctgttaatgtttgcctatgagggcaaaagaagcttttcctt
ttcaatttcctatgtaatgttactctctgctagtgactttggctgttggtgggttgccggaagatgcaattcaacaaaagttggtgggctgcaggagaga
atgccccagacgcccaccctcgtagcaatgtttgacagcagcagtttccatcggaacttgtatcagtccaaagaagaaagttgttctgaactgtattacc
aagataacaacctgctttctgggtctctggaagcacttatccaacacttagtgccaaacgtcgattactatccggatagaacatacatcttcaccttctt
gctgagctccagactttttattcacccatctgagttgatggccagagtctgccatgtatgtatggagcagcagcgactgaatgagccagggttggataag
ttgacgaaaaaaagtcacaactttttcgagatttactatgcaacanaagtgcgacaattttgaatctgaaataacaccatctaaaacttgtcaagatcat
gtataagtttttggttgatgtccccctttcctggaaggagaggattatcatgcgggcagacaaaaaaagccaagtgcgaaaaaatt
3' EST: DT535047 [+]gcacagaccatgtttaataaatacaaaaaacaattcacaaaataaatttaaattacaaaagaaacaaacaaggaattgtgtaaacagctaaaaaaagacc
attggaagatcagtccagtgatatccttgcagtgaggtgcttttctatcttcttttcaaagggagatagtttatgcattggcaattattactgataatac
tggtaagtcacattgctagcccagtttactatccagcagtaaacttaagcttgaacaatagaataaacctggacagtatgaaacggatataaaccaccgc
cagcgagtggtttttattttttaaatgtctttgcttttccctcaggcaaaattcctggtgttaacagctttattttttatacagacatttttagatgatc
aaggtcaatgggactggctagcagaatgcaacatccagcaaataatctaattataagccacgcatgagaaaaaaaatctcagcacaagggtggctgtgaa
tgattaagtctttattgaccttaacaagcatgctaggccagtcagtggaaattcttctgcttgtgtgcactcttagctctcttgatttaccaatgctggg
ctccaatctcggctgcctgctctgttgccatcaagtgcaaaaagggtccataaatgataacaattttctggcaggtcattgacacattatgctctgccca
aaagcgctgacctcagagtcttccatctgtctttttcaatatgattctcaggaccttcactctcataggaagccaagtaaagtgcatcctcactgaaaat
gggtgcggtcagcacataatgtaatattttccgatccttttcaaatggacactcaacttgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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