XB-CLONE-3053137
Clone Name: IMAGE:7849497
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535096 [+]
agggccgcactctacagcacccgctctcagatagtagcaccatgcctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgc
caaacgcattgtggccactggcaagggcatcctggcagccgatgagtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgag
gagaaccgccgcttataccgccagctgctcttcaccgccgacgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccaca
agagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaa
cggtgaaaccacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaag
atctcagaacacactccctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattg
tggagcccgaggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgacca
ccacatctacctggaagggaccctgctgaaaccaaacatggttacccccgggcatgcctgcaccaagaagtacccccctcaggaggttgctatggcaaca
gtcaccgccctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggtcagagtg
3' EST: DT535095 [+]gggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgtagcccggag
acagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcagtctctctct
ctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcactaggca
aatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctctccgc
tggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccccaggc
tttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacggtggcc
tcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctcctgag
gggggtacttcttggtgcaggcatgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttgtacac
tgcagccagggacttctctgtcacatactggcacctcttcaggtcatggtctccatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: